Sample can be neutralized and maintained in stable condition by putting neutral solution in tube when recovering anti-body.
As shown below, the anti-body concentrations were determined quantitatively from medium of CHO cells. The purified antibodies show very less impurities by the results from electrophoresis.
The monolithic silica phase used in MonoSpin columns is state-of-the-art in stationary phase technology allowing high recovery and adsorption and desorption performance. The structure of the silica allows for less obstruction of the binding proteins compared to conventional particle based phases allowing for much higher efficiency of separation.
MonoSpin ProA can achieve a 90% recovery rate of IgG using only 100 µL of elution buffer compared other other brands requiring 400 µL of buffer while only achieving a recovery rate of 70%.
MonoSpin ProA's high manufacturing standards allows for uniform performance across wells in a 96 well plate format making MonoSpin ProA ideal for batch process.
Absorbance of MonoSpin ProA across wells. Sample volume 150 µL, elution volume 150 µL, recovery rate 90% (CV 3.1%), and IgG concentration 1.3 mg/mL.
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MonoSpin ProA spin column L Type 4 pcs.