InertSustain AX-C18

InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by Anion Exchange group works. By controlling the amount of Anion Exchange group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS.

Specifications

• Base Material : High Purity ES Silica Gel
• Particle Size : 3 μm, 5 μm
• Surface Area : 200 m2/g
• Pore Size : 200 Å (20 nm)
• Pore Volume : 1.00 mL/g
• Functional Group : Octadecyl + Alkylamino (tertiary amine)
• End-capping : Yes
• Carbon Loading : 8.0 %
• USP Code : L1, L78
• pH Range : 1 - 9

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Overview

Retention behavior that combines both reversed phase and anion exchange

InertSustain AX-C18 Anion Exchange Column has increased retention of acidic compounds/decreased retention of basic compounds compared to typical C18 columns.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of nucleobases and monophosphates graph

Compounds:

1. Cytosine (basic) 2. Uracil (neutral) 3. CMP (acidic) 4. UMP (acidic) 5. Adenine (basic) 6. AMP (acidic)

InertSustain AX-C18 Anion Exchange Column HPLC Columns compounds 2

Excellent retention for acidic compounds

No ion pair reagent in the eluent means highly polar acidic compounds are retained.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of sugar-nucleotides

Strongly retains highly polar acidic compounds which normally cannot be retained by C18 columns for analysis of highly polar compounds!

InertSustain AX-C18 Anion Exchange Column strongly retains acidic compounds even if the salt concentration of the eluent is low. Also compatible with MS analysis.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of monophosphoric acid

Strongly retains highly polar acidic compounds even when salt concentration is as low as 10 mM! Sharp elution peaks!

Can be analyzed at low salt concentration. Compatibility with MS!

Excellent reproducibility and quality

Reproducibility issues with mixed mode are solved by GL Sciences' original packing and manufacturing technology.

InertSustain AX-C18 Anion Exchange Column HPLC Columns lot to lot variation graph