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HPLC Columns
GL Sciences columns provide excellent separation and resolution of target compounds featuring new high-purity evolved surface silica. GL Sciences columns silica base is uniquely modified to provide maximum column performance including low operating back-pressure, high inertness, high efficiency and wide solvent compatibility. Get the appropriate column from a large selection of column configurations and formats compatible with many HPLC methods including analytical, preparative, capillary HPLC and UHPLC. Read more
For specialized analysis of peptides, nucleic acids, and antibodies.
Inertsil ODS-3 is still GL Sciences’ most popular phase and continues to be used widely and reliably for long-established methods in pharmaceutical, and contract research labs. Inertsil ODS-3 columns have a relatively strong retentivity compared to commercially available ODS columns. In addition, the introduction of higher surface area si...
From $244.00
Inertsil ODS-3V offers all of the outstanding chromatographic benefits of Inertsil ODS-3 with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance. Each Inertsil ODS-3V is delivered with a Manufacturer‘s Validation Certificate showing the detailed results of every QA ...
From $640.00
InertSustain C30 is an HPLC column in which triacontyl groups are chemically bonded to silica gel. This column has high stereoselectivity and excellent separation ability for isomers and structurally similar compounds with high lipophilicity. Specifications • Base Material : High Purity ES Silica Gel • Particle Size : 3 μm, 5 μm •...
From $244.00
Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C18 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise con...
From $244.00
InertSustain AQ-C18 columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goals in developing reversed phase methods. The optimization of bonding of the C18 groups at equal distance to the silica gel enable InertSustain AQ-C18 columns to offer significant retention for highly polar comp...
From $244.00
Increasing Efficiency, Throughput and Productivity InertSustainSwift C18 columns are designed to deliver rapid separations with symmetric peaks to minimize analysis time as much as possible. InertSustainSwift C18 is also ideal for LC-MS and LC-MS/MS methods which offer highly sensitive results and enables MS compatible buffers to be u...
From $244.00
InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by Anion Exchange group works. By controlling the amount of Anion Exchange group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS. Specificat...
From $244.00
The High Performance Core-shell Column For UHPLC/HPLC Specifications • Base Material: Core-shell type silica gel • Particle Size: 2.6 µm • Solid Core: 2.0 µm • Surface Area: 200 m2/g • Pore Size: 90 Å (9 nm) • Functional Group: Octadecyl • End-capping: Yes • Carbon Loading: 15% • USP Code: L1 • pH Range: 1 - 10 •...
From $380.00
Designed for advanced protein analysis, the ProteoSil 300-C18 column offers the ideal capabilities when enhanced retention and sample load are critical for C4 or C8 columns.
From $202.00
Ideal for mid-sized molecules, the ProteoSil 200-C18 column allows for separation across 100Å pore columns and is easy to clean for faster analysis.
From $202.00
Offering high inertness and durability, the ProteoSil 100-C18 is perfect for analyzing low-molecular-weight compounds, enhancing sensitivity in LC-MS applications.
From $309.00
The 200 Å pore size silica creates the opportunity to separate compounds having a molecular weight from small to large molecules. In addition, the usage of highly inert packing material packed into a new Steel-Coated-PEEK hardware deliver excellent peak shapes for various analytes without any adsorption. Specifications • Base Material...
From $1,150.00
Ultra High Retentivity Ideal for Separation of Basic Molecules & its Related Substances, Process Impurities Inertsil ODS-HL employs a highly inert packing material which provides pure hydrophobic interaction between analytes without generating any secondary interaction delivering sharp peaks. High-Density bonding of C18 phase delivers ...
From $244.00
Inertsil ODS-4 delivers the same extreme inertness to any type of compounds just like InertSustain C18 along with unprecedented stability under 100 % aqueous mobile phases for qualitative and quantitative analysis. However, as the base silica gel and carbon loading are different on Inertsil ODS-4, differences in selectivity can be observe...
From $244.00
Inertsil ODS-4 columns have proven superior worldwide for analysis of strong pharmaceutical bases, acids, chelating compounds, and zwitterions. The long-awaited validated Inertsil ODS-4V has now been added to our product lineup. Each Inertsil ODS-4V is delivered with a Manufacturer’s Validation Certificate showing the detailed results ...
From $670.00
Inertsil ODS-SP is super base deactivated and optimally bonded to retain hydrophilic compounds without excessive retention of hydrophobic compounds achieving better separations faster than before. As the carbon load of Inertsil ODS-SP is relatively low, it is compatible with 100 % aqueous eluents and offer faster equilibration of column f...
From $244.00
GL Sciences offers a polymerically bonded ODS-P phase which provide high steric selectivity for separation of planar and non-planar compounds. nertsil ODS-P columns are also ideal for the HPLC analysis of 16 PAH compounds, listed as target pollutants by the US EPA. Specifications • Silica : 3 Series High Purity Silica Gel • Particle ...
From $244.00
Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C18 group. The embedded polar group makes the C18 phase stable in 100 % aqueous eluents without "phase collapse." This phase is also extremely "base deactivated" and provides superior peak shape for acids and bases in organic eluents as well a...
From $244.00
Inertsil WP300 C18 (wide pore size of 300 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible separations of proteins and peptides. The results of GL Sciences’ original end-capping technique provide high efficiency and good peak shape for proteins an...
From $244.00
Inertsil ODS-2 columns have a pore size of 150 Å offering symmetric peaks for bases, acids with low pressure. When Inertsil ODS-2 was introduced in 1987s, this HPLC transformed the entire industry. Inertsil ODS-2 was the first HPLC phase created using ultra high purity silica, which produced superior base deactivation. Inertsil ODS-2 is o...
From $280.00
Inertsil ODS columns are general purpose, reversed phase C18 columns available in 5 μm and 10 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 1986. We recommend InertSustain C18 or Inertsil ODS-4 columns for all new method development. Specifications • Silica : Spherical Silica Gel • Parti...
From $280.00
InertSustain C8 is an octyl group (C8) bonded column delivering the same extreme inertness to any type of compounds just like InertSustain C18, which enables rapid analysis of highly hydrophobic compounds delivering symmetric peaks at a wide range of pH. Specifications • Silica : High Purity ES Silica Gel • Particle Size : 2 μm, 3 μm...
From $244.00
InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C18, which is ideal for analyzing low polarity analytes. In addition, the optimized 200Å pore size silica enables to analyze and retain peptides and oligonucleotides which have a molecular weig...
From $244.00
Many chromatographers prefer a C8 column when an ODS phase shows excessive retention values. Inertsil C8-4 provides the same separation pattern (selectivity) and extreme inertness to any type of compounds just like Inertsil ODS-4, which enables easy method transfer from ODS-4 to C8-4 while other commercially available ODS and C8 columns c...
From $244.00
The same base silica gel and bonding technology that is used for Inertsil ODS-3 is also employed for Inertsil C8-3. The difference between the two phases is just the length of the hydrocarbon ligands. 10 μm particle size columns are also available to meet the requirement of various pharmacopeia methods. We recommend InertSustain C8 column...
From $244.00
Optimized for proteins and peptides, the ProteoSil 300-C8 ensures unique selectivity and hydrophobicity compared to shorter-chain C4 phases, suitable for protein and hydrophobic peptide separations.
From $202.00
Specialized for the analysis of mid-sized molecules, the ProteoSil 200-C8 shows superior retention for analytes that are strongly retained by C18 columns
From $319.00
Inertsil C8 columns have a pore size of 150 Å and it is recommended for rapid analysis of highly hydrophobic compounds. We recommend InertSustain C8 or Inertsil C8-4 columns for all new method development. Specifications • Silica : 2 Series High Purity Silica Gel • Particle Size : 5 μm • Surface Area : 320 m2/g • Pore Size : 150 ...
From $280.00
Tailored for the analysis of proteins and peptides, especially hydrophobic peptides, the ProteoSil 300-C4 facilitates shorter retention times and is ideal for proteins with moderate retention needs.
From $202.00
Inertsil C4 columns have a pore size of 150 Å and is recommended for rapid analysis of highly hydrophobic compounds such as fat-soluble vitamins. Specifications • Silica : 2 Series High Purity Silica Gel • Particle Size : 5 μm • Surface Area : 320 m2/g • Pore Size : 150 Å (15 nm) • Pore Volume : 1.20 mL/g • Functional Group : But...
From $280.00
Inertsil WP300 C8 (wide pore size of 300 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible rapid separations of proteins and peptides. Inertsil WP300 C8 delivers rapid analysis with sharper peaks compared to Inertsil WP300 C18. Specifications •...
From $244.00
Inertsil WP300 C4 is a butyl group bonded phase utilizing silica gel with wide pores (300 Å). Also, an optimal polar group is embedded between silica surface and butyl group, which reduces adsorption of basic compounds and fat-soluble proteins. Inertsil WP300 C4 is recommended for the analysis of large, highly fat-soluble proteins and pep...
From $244.00
InertSustain PFP columns are bonded with pentafluorophenyl groups to our newly developed ES silica gel, which delivers unique separation patterns with excellent peak shape and sensitivity. The chromatographic difference stems from the fact that the PFP phases provide interactions such as π-π, dipole, hydrogen bonding, and ionic interactio...
From $244.00
InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6- carbon) linker and is densely bonded to our newly developed ES silica gel delivering complementary selectivity to straight alkyl-chain columns, but with industry leading inertness, lot-to-lot reproducibility and low back press...
From $244.00
InertSustain Phenyl delivers an extremely unique reverse phase characteristics that are critical to resolving compounds that could not be separated on a C18 or C8 phase. InertSustain Phenyl provides not only pi-pi interactions, but also hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. A...
From $244.00
Just like InertSustain Phenyl, Inertsil Ph-3 has phenyl groups directly bonded to the silica gel which provides pure reverse phase characteristics that are critical to resolving highly polar compounds like acidic and basic pharmaceuticals. The near perfect phenyl phase coverage on this material results in symmetric, narrow peaks for even ...
From $244.00
Inertsil Ph has phenethyl groups bonded to silica gel which offers weak pi-pi interactions. As it is modified with phenethyl groups, hydrophobic interactions between alkyl chain and analytes play an important role in separation as well as π-π interactions. To change the selectivity or elution pattern drastically, InertSustain Phenyl is re...
From $280.00
In general, the stability and reproducibility of the Cyano phase available in the market are poor. Many batch-to-batch or lot-to-lot reproducibility issues are occurring at many laboratories. The InertSustain Cyano columns were developed to resolve these problems and are designed using the most modern LC column technology available provid...
From $244.00
InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phase...
From $244.00
Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime. ...
From $244.00
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when...
From $244.00
Modified with amide groups, the ProteoSil HILIC column excels in the separation of highly hydrophilic compounds, peptides, glycans, and oligonucleotides.
From $202.00
InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of ...
From $244.00
Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous ana...
From $244.00
Equipped with dihydroxypropyl groups bonded to silica gel, the ProteoSil 300-SEC column is suited for the analysis of large molecules and can be used as a dial column.
From $202.00
Constructed for aqueous size-exclusion chromatography, the ProteoSil 100-SEC can handle higher operating pressures and is suitable for series column configurations.
From $221.00
Inertsil Diol column has characteristics of dihydroxypropyl group bonded phase. It shows unique selectivity normal phase mode. The separation mechanism of diol column is featured by hydrogen bonding interactions between diol groups and polar compounds. Diol columns provide an alternative selectivity to silica columns often with increased ...
From $244.00
Inertsil SIL-100A is a pure silica gel column available in normal phase mode. Because of the high quality of its silica gel, Inertsil SIL-100A achievs separation with sharp peaks and provides high column-to-column reproducibility. This excellent silica gel ideally designed for HPLC is the basis for “Inertsil 3-series” of GL Scineces. GL S...
From $244.00
Non-polar phases including alkyl phases such as C18, C8, C4 and other functionalities such as phenyl and cyano groups. GL Sciences provides a diverse range of reverse phases for different analyte retentivities and selectivities by group modification. A high degree of analyte targeting can be achieved using our selection of reverse phases.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| InertSustain C18 | First choice with ultra high inertness and high durability | L1 | 2, 3, 5, 10 | 10 | 350 |
| InertSustain AQ-C18 | First choice for high polar compounds | L1, L96 | 1.9, 3, 5 | 10 | 350 |
| InertSustainSwift C18 | First analysis with ultra high inertness and high durability | L1 | 1.9, 3, 5 | 20 | 200 |
| InertSustain AX-C18 | Analysis of anionic highly polar compounds | L1, L78 | 3, 5 | 20 | 200 |
| InertCore Plus C18 | Ideal for analyses requiring a high number of theoretical stages | L1 | 2.6 | 9 | 200 |
| Inertsil ODS-HL | Ultra high retentivity, High-density bonding of C18 phase | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-4 | Ultra high inertness, High plate count, Medium retentivity | L1 | 2, 3, 5 | 10 | 450 |
| Inertsil ODS-4V | Inertsil ODS-4 Validated column | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-3 | Strong retentivity, Lower column backpressure, Very inert | L1 | 2, 3, 4, 5, 10 | 10 | 450 |
| Inertsil ODS-3V | Inertsil ODS-3 Validated column | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-SP | Weak retentivity, for hydrophobic compounds | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-P | High steric selectivity | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-EP | A polar functional group embedded | L1 | 5 | 10 | 450 |
| Inertsil WP300 C18 | Analysis of high molecules | L1 | 5 | 30 | 150 |
| Inertsil ODS-2 | Ultra pure silica gel is used | L1 | 5 | 15 | 320 |
| Inertsil ODS | Inertness 1st generation | L1 | 5, 10 | 10 | 350 |
| InertSustain C8 | First choice with ultra high inertness and high durability | L7 | 2, 3, 5 | 10 | 350 |
| InertSustainSwift C8 | High inertness and high durability C8 column | L7 | 1.9, 3, 5 | 20 | 200 |
| Inertsil C8-4 | Ultra high inertness, High plate count, Low retentivity | L7 | 2, 3, 5 | 10 | 450 |
| Inertsil C8-3 | Strong retentivity, Lower column backpressure, Very inert | L7 | 2, 3, 5, 10 | 10 | 450 |
| Inertsil C8 | Ultra pure silica gel is used | L7 | 5 | 15 | 320 |
| Inertsil C4 | Low retentivity | L26 | 5 | 15 | 320 |
| Inertsil WP300 C8 | Suitable for high molecules | L7 | 5 | 30 | 150 |
| Inertsil WP300 C4 | Suitable for high molecules | L26 | 5 | 30 | 150 |
| InertSustain PFP | Extremely Strong retention of highly polar basic compounds | L43 | 3, 5 | 10 | 350 |
| InertSustain Phenylhexyl | Strong π-π interactions and hydrophobic interactions | L11 | 3, 5 | 10 | 350 |
| InertSustain Phenyl | Extremely strong π-π interactions | L11 | 2, 3, 5 | 10 | 350 |
| Inertsil Ph-3 | Strong π-π interactions | L11 | 2, 3, 5 | 10 | 450 |
| Inertsil Ph | High inertness, Weak π-π interactions | L11 | 5 | 15 | 320 |
| InertSustain Cyano | Ultra inertness and can be used in reversed phase mode | L10 | 3, 5 | 10 | 350 |
Phases providing high retentivity of polar compounds in immobilized water layers on the stationary phase surface in hydrophilic interaction liquid chromatography (HILIC) mode. Our choice of funtionalities allow for an excellent spread of different separation patterns for the analysis of compounds such as sugars.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| InertSustain Amide | First choice for HILIC mode | L68 | 3, 5 | 10 | 350 |
| Inertsil HILIC | Separation of highly polar basic compounds | L20 | 3, 5 | 10 | 450 |
| InertSustain NH2 | First choice for sugar analysis | L8 | 3, 5 | 10 | 350 |
| Inertsil NH2 | Sugar analysis, High retentive in normal phase mode | L8 | 3, 5 | 10 | 450 |
| Inertsil Amide | Increasing retentivity of high polar compounds | L68 | 3, 5 | 10 | 450 |
Phases with ionizable functional groups having high ion exchange capacities are available for both anion and cation exchange chromatography. We feature popular groups for wide method compatibility. In addition, mix-mode columns featuring alkyl groups are available for alternate selectivity.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| Inertsil AX | Anion-exchange column | ― | 5 | 10 | 450 |
| Inertsil CX | Cation-exchange column | L9 | 5 | 10 | 450 |
| InertSustain AX-C18 | Analysis of anionic highly polar compounds | L1, L78 | 3, 5 | 20 | 200 |
High performance phases for retentivity of polar compounds. Our normal phases are available a wide range of pore sizes (10-30 nm) for excellent choice in retentivity and selectivity in analysis of up to large molecules.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| Inertsil Diol | First choice for normal phase mode, For SEC | L20 | 3, 5 | 10 | 450 |
| Inertsil SIL-100A | Ultra pure silica gel with 100Å pore size | L3 | 3, 5 | 10 | 450 |
| Inertsil SIL-150A | Ultra pure silica gel with 150Å pore size | L3 | 5 | 15 | 320 |
| Inertsil WP300 SIL | Ultra pure silica gel with 300Å pore size | L3 | 5 | 30 | 150 |
| Inertsil CN-3 | Can be also used in reversed phase mode | L10 | 3, 5 | 10 | 450 |
| InertSustain Cyano | Ultra inertness and can be used in reversed phase mode | L10 | 3, 5 | 10 | 350 |
Columns featuring diol phases in convenient pore sizes that are compatible with aqueous (GFC) or organic (GPC) size-exclusion chromatography. Our columns are available in smaller inner diameters for solvent saving, low-cost analysis. Serial coupling using different GL Sciences SEC columns allows for analysis of a broad range of molecular weights.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| Inertsil WP300 Diol | High molecule SEC, Can be also used in normal phase mode | L20, L33 | 5 | 30 | 150 |
| Inertsil Diol | First choice for normal phase mode, For SEC | L20 | 3, 5 | 10 | 450 |
HPLC Column Selection Guide
Separation Mode Selection for Analyte Polarity
Column Selection for Analyte Properties
Column Selection for Vitamins
Column Selection for Sugars and Nucleic acids
Column Selection for Medicines
Column Selection for Organic acids
Column Selection for Amino acids
Column Selection for Proteins and Peptides
Column Selection for Pesticides, Veterinary medicines, Aldehydes and Polymers
HPLC Columns Resources:
- Download GL Sciences 2022 HPLC Columns Catalog — Download
- Download GL Sciences 2022 InertSustain Catalog— Latest HPLC Column Technology Download
- Search Application Notes For HPLC Columns — Search Applications Database
- HPLC Columns’ Specification — Summary of GL Sciences HPLC Column Specifications. Learn More
- HPLC Column Selection Overview — Separation mode selection for analyte polarity & HPLC column selection for analyte properties. Learn More
- HPLC Column Selection By USP — Recommended GL Sciences HPLC Columns based on USP Code. Learn More
- HPLC Column End Fittings — Summary of GL Sciences' end-fitting style & recommended operating pressure. Learn More
- Reversed Phase HPLC Column Selection Guide— Download Reverse Phase HPLC Column Selection Guide. Learn More
- Normal Phase Selection Guide — Download Normal Phase HPLC Column Selection Guide. Learn More
- How to Select a Guard Column — Download guide to select the right guard column. Learn More
- Choosing a Preparative Column — Download Guide to select the right preparative column. Learn More