GL Sciences’ MonoCap series, created synthetically via sol-gel method, and an octadecyl silane chemically bonded, has a very uniform three dimensional structure that shows excellent reproducibility from batch-to-batch. The solid structure of GL Sciences’ monolithic silica eliminates the need for frits or filters at the ends of the column, thereby reducing dead volume that might otherwise lead to band broadening or sample recovery. The high porosity of our monolithic silica allows high flow rates to be used without loss of resolution or creation of high operating pressure. An optimized balance of throughpores and mesopores provides the critically important combination of efficiency, separation speed, large volume sample-loading, and small volume sample-recovery.
Amide groups are chemically bonded to the monolithic silica and makes it suitable for the analysis of sugars via HILIC mode. As the back pressure is significantly low, a 500 mm length MonoCap Amide column deliver over 40,000 plates offering high efficiency. Generally, HILIC mode uses acetonitrile at a concentration between 65-95 % in an a...
Workable at a broad range of linear velocity from 0.5 to 5 mm/s without sacrificing efficiency and separation at high speed. The number of theoretical plates produced by MonoCap C18 Fast-flow is nearly equivalent to a totally porous particle type capillary column packed with a 5µm packing material. Columns are protected by either metal or...
Maximizing all the benefits and advantages of monolithic technology, MonoCap High Resolution and High Resolution Ultra are appropriate for the efficient separation of peptides and protein digests. MonoCap High Resolution provide extremely high efficiency, delivering over 200,000 plates for a 2,000 mm length column. The High Resolution...
MonoCap C18 Nano-flow produces higher number of theoretical plates compared to a totally porous particle type capillary column packed with a 3µm packing material. It can be operated at a wide range of flow rate with low back pressure and achieve very high sensitive results in Nano-LC-ESI/MS applications. Columns are protected by either me...
MonoCap Trap columns have a relatively big throughpore and workable at a high flow rate such as 10 μL/min. This benefit makes MonoCap Trap columns to be appropriate for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection. End-fittings are 1/16“ (10-32 UNF). 1/32“...
The MonoCap C18 Fast-flow is also available in 0.5 mm I.D. size, which can be used at a wide range of flow rate from 6 to 100 L/min without sacrificing efficiency. The number of theoretical plates produced by MonoCap C18 WideBore is nearly equivalent to a totally porous particle type capillary column packed with a 5µm packing material. Co...
MonoCap SCX is bonded with benzene sulfonic acid groups (strong cation exchange) and appropriate for 2D LC applications for the separation of biomolecules such as peptides and proteins.
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