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Reversed Phase HPLC Columns

Experience a wide selection of phases for reversed phase HPLC with GL Sciences reversed phase columns. Select from phases including C18, C8, C4, phenyl and cyano functionalized groups for distinct analyte selectivity. GL Sciences reversed phase columns are available in many sizes and formats featuring our high-purity silica and end-capping. Read more

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InertSustain C30 columns
InertSustain C30
InertSustain C30 is an HPLC column in which triacontyl groups are chemically bonded to silica gel. This column has high stereoselectivity and excellent separation ability for isomers and structurally similar compounds with high lipophilicity. Specifications • Base Material : High Purity ES Silica Gel • Particle Size : 3 μm, 5 μm •...
From $191.00
InertSustain C18 HPLC Columns
InertSustain C18 HPLC Columns
Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C18 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise con...
From $191.00
InertSustain AQ-C18 HPLC Columns
InertSustain AQ-C18 HPLC Columns
InertSustain AQ-C18 columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goals in developing reversed phase methods. The optimization of bonding of the C18 groups at equal distance to the silica gel enable InertSustain AQ-C18 columns to offer significant retention for highly polar comp...
From $191.00
Picture of InertSustainSwift C18 HPLC Column, InertCoat Hardware, 1.9 µm, 50 x 2.1 mm
InertSustainSwift C18 HPLC Column, InertCoat Hardware, 1.9 µm, 50 x 2.1 mm
SKU# 5020-32012
$982.00
Picture of InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, 2/Pk
InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, 2/Pk
SKU# 5020-32038
$528.00
Picture of InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 3 µm, 100 x 2.1 mm
InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 3 µm, 100 x 2.1 mm
SKU# 5020-32010
$982.00
Picture of InertSustainSwift C18 HPLC Column, InertCoat Hardware, 3 µm, 100 x 2.1 mm
InertSustainSwift C18 HPLC Column, InertCoat Hardware, 3 µm, 100 x 2.1 mm
SKU# 5020-32016
$982.00
Picture of InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, 2/Pk
InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, 2/Pk
SKU# 5020-32040
$500.00
Picture of InertSustain C18 HPLC Column, InertCoat Hardware, 3 µm, 50 x 2.1 mm
InertSustain C18 HPLC Column, InertCoat Hardware, 3 µm, 50 x 2.1 mm
SKU# 5020-32003
$937.00
Picture of InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, 2/Pk
InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, 2/Pk
SKU# 5020-32034
$528.00
Picture of InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, Holder Set
InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, Holder Set
SKU# 5020-32041
$691.00
Picture of MonoCap C18 High Resolution HPLC Column, 750 x 0.05 mm
MonoCap C18 High Resolution HPLC Column, 750 x 0.05 mm
SKU# 5020-10103
$2,873.00
Picture of InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 3 µm, 50 x 2.1 mm
InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 3 µm, 50 x 2.1 mm
SKU# 5020-32009
$937.00
Picture of InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 3 µm, 150 x 2.1 mm
InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 3 µm, 150 x 2.1 mm
SKU# 5020-32011
$1,037.00
Picture of InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, 2/Pk
InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, 2/Pk
SKU# 5020-32036
$500.00
Picture of InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, Holder Set
InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, Holder Set
SKU# 5020-32037
$691.00
Picture of InertSustain C18 HPLC Column, InertCoat Hardware, 2 µm, 50 x 2.1 mm
InertSustain C18 HPLC Column, InertCoat Hardware, 2 µm, 50 x 2.1 mm
SKU# 5020-32000
$982.00
Picture of InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 1.9 µm, 150 x 2.1 mm
InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 1.9 µm, 150 x 2.1 mm
SKU# 5020-32008
$1,164.00
Picture of MonoTower C18 Cartridge, 50 x 3.0 mm
MonoTower C18 Cartridge, 50 x 3.0 mm
SKU# 5020-10860
$400.00
Picture of InertSustainSwift C18 HPLC Column, InertCoat Hardware, 1.9 µm, 150 x 2.1 mm
InertSustainSwift C18 HPLC Column, InertCoat Hardware, 1.9 µm, 150 x 2.1 mm
SKU# 5020-32014
$1,164.00
Picture of InertSustainSwift C18 HPLC Column, InertCoat Hardware, 3 µm, 50 x 2.1 mm
InertSustainSwift C18 HPLC Column, InertCoat Hardware, 3 µm, 50 x 2.1 mm
SKU# 5020-32015
$937.00
Picture of InertSustain C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, 2/Pk
InertSustain C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, 2/Pk
SKU# 5020-32032
$500.00
Picture of InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, Holder Set
InertSustain AQ-C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, Holder Set
SKU# 5020-32035
$719.00
Picture of InertSustainSwift C18 HPLC Column, InertCoat Hardware, 1.9 µm, 100 x 2.1 mm
InertSustainSwift C18 HPLC Column, InertCoat Hardware, 1.9 µm, 100 x 2.1 mm
SKU# 5020-32013
$1,046.00
Picture of InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, Holder Set
InertSustainSwift C18 InertCoat Hardware Guard Cartridge, 1.9 µm, 10 x 2.1 mm, Holder Set
SKU# 5020-32039
$719.00
Picture of InertSustain C18 HPLC Column, InertCoat Hardware, 3 µm, 150 x 2.1 mm
InertSustain C18 HPLC Column, InertCoat Hardware, 3 µm, 150 x 2.1 mm
SKU# 5020-32005
$1,037.00
Picture of InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 1.9 µm, 50 x 2.1 mm
InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 1.9 µm, 50 x 2.1 mm
SKU# 5020-32006
$982.00
Picture of InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 1.9 µm, 100 x 2.1 mm
InertSustain AQ-C18 HPLC Column, InertCoat Hardware, 1.9 µm, 100 x 2.1 mm
SKU# 5020-32007
$1,046.00
Picture of InertSustain C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, Holder Set
InertSustain C18 InertCoat Hardware Guard Cartridge, 3 µm, 10 x 2.1 mm, Holder Set
SKU# 5020-32033
$691.00
Picture of InertSustain C18 HPLC Column, InertCoat Hardware, 2 µm, 100 x 2.1 mm
InertSustain C18 HPLC Column, InertCoat Hardware, 2 µm, 100 x 2.1 mm
SKU# 5020-32001
$1,046.00
Picture of InertSustain C18 HPLC Column, InertCoat Hardware, 2 µm, 150 x 2.1 mm
InertSustain C18 HPLC Column, InertCoat Hardware, 2 µm, 150 x 2.1 mm
SKU# 5020-32002
$1,164.00
Picture of InertSustainSwift C18 HPLC Column, InertCoat Hardware, 3 µm, 150 x 2.1 mm
InertSustainSwift C18 HPLC Column, InertCoat Hardware, 3 µm, 150 x 2.1 mm
SKU# 5020-32017
$1,037.00
Picture of InertSustain C18 InertCoat Hardware Guard Cartridge, 2 µm, 10 x 2.1 mm, 2/Pk
InertSustain C18 InertCoat Hardware Guard Cartridge, 2 µm, 10 x 2.1 mm, 2/Pk
SKU# 5020-32030
$528.00
Picture of MonoTower C18 Cartridge, 150 x 3.0 mm
MonoTower C18 Cartridge, 150 x 3.0 mm
SKU# 5020-10862
$500.00
Picture of InertSustain C18 HPLC Column, InertCoat Hardware, 3 µm, 100 x 2.1 mm
InertSustain C18 HPLC Column, InertCoat Hardware, 3 µm, 100 x 2.1 mm
SKU# 5020-32004
$982.00
Picture of MonoCap C18 High Resolution HPLC Column, 750 x 0.05 mm
MonoCap C18 High Resolution HPLC Column, 750 x 0.05 mm
SKU# 5020-10003
$2,873.00
Picture of MonoTower C18 Cartridge, 100 x 3.0 mm
MonoTower C18 Cartridge, 100 x 3.0 mm
SKU# 5020-10861
$446.00
Picture of InertSustain C18 InertCoat Hardware Guard Cartridge, 2 µm, 10 x 2.1 mm, Holder Set
InertSustain C18 InertCoat Hardware Guard Cartridge, 2 µm, 10 x 2.1 mm, Holder Set
SKU# 5020-32031
$719.00
Picture of MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.2 mm
MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.2 mm
SKU# 5020-10023
$2,873.00
Picture of MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.2 mm
MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.2 mm
SKU# 5020-10123
$2,873.00
Picture of MonoClad C18-HS, 100 x 3.0 mm
MonoClad C18-HS, 100 x 3.0 mm
SKU# 5020-10802
$928.00
Picture of MonoClad C18-HS, 50 x 3.0 mm
MonoClad C18-HS, 50 x 3.0 mm
SKU# 5020-10801
$837.00
Picture of MonoClad C18-HS, 150 x 3.0 mm
MonoClad C18-HS, 150 x 3.0 mm
SKU# 5020-10803
$1,046.00
Picture of MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.1 mm
MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.1 mm
SKU# 5020-10013
$2,873.00
Picture of MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.1 mm
MonoCap C18 High Resolution 750 HPLC Column, 750 x 0.1 mm
SKU# 5020-10113
$2,873.00
Picture of MonoClad C18-HS, 250 x 3.0 mm
MonoClad C18-HS, 250 x 3.0 mm
SKU# 5020-10804
$1,191.00
InertSustainSwift C18
InertSustainSwift C18
Increasing Efficiency, Throughput and Productivity InertSustainSwift C18 columns are designed to deliver rapid separations with symmetric peaks to minimize analysis time as much as possible. InertSustainSwift C18 is also ideal for LC-MS and LC-MS/MS methods which offer highly sensitive results and enables MS compatible buffers to be u...
From $191.00
InertSustain AX-C18
InertSustain AX-C18
InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by Anion Exchange group works. By controlling the amount of Anion Exchange group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS. Specificat...
From $191.00
InertCore Plus C18
InertCore Plus C18
The High Performance Core-shell Column For UHPLC/HPLC Specifications • Base Material: Core-shell type silica gel • Particle Size: 2.6 µm • Solid Core: 2.0 µm • Surface Area: 200 m2/g • Pore Size: 90 Å (9 nm) • Functional Group: Octadecyl • End-capping: Yes • Carbon Loading: 15% • USP Code: L1 • pH Range: 1 - 10 •...
From $455.00
Inertsil ODS-HL
Inertsil ODS-HL
Ultra High Retentivity Ideal for Separation of Basic Molecules & its Related Substances, Process Impurities Inertsil ODS-HL employs a highly inert packing material which provides pure hydrophobic interaction between analytes without generating any secondary interaction delivering sharp peaks. High-Density bonding of C18 phase delivers ...
From $191.00

Reversed Phase Column Specification

 
ColumnFeaturesUSP CodeParticle Size (µm)Pore Size (nm)Surface Area (m2/g)Carbon LoadingEnd-CappingInertness
Recommended pH range
InertSustain C18First choice with ultra high inertness and high durabilityL12, 3, 5, 101035014Yes★★★★★1 - 10
InertSustain AQ-C18First choice for high polar compoundsL1, L961.9, 3, 51035013Yes★★★★★1 - 10
InertSustainSwift C18First analysis with ultra high inertness and high durabilityL11.9, 3, 5202009Yes★★★★★1 - 10
InertSustain AX-C18Analysis of anionic highly polar compoundsL1, L783, 5202008Yes★★★★1 - 9
InertCore Plus C18Ideal for analyses requiring a high number of theoretical stagesL12.6920015Yes★★★★1 - 10
Inertsil ODS-HLUltra high retentivity, High-density bonding of C18 phaseL13, 51045023Yes★★★★★2 - 7.5
Inertsil ODS-4Ultra high inertness, High plate count, Medium retentivityL12, 3, 51045011Yes★★★★★2 - 7.5
Inertsil ODS-4VInertsil ODS-4 Validated columnL13, 51045011Yes★★★★★2 - 7.5
Inertsil ODS-3Strong retentivity, Lower column backpressure, Very inertL12, 3, 4, 5, 101045015Yes★★★★2 - 7.5
Inertsil ODS-3VInertsil ODS-3 Validated columnL13, 51045015Yes★★★★2 - 7.5
Inertsil ODS-SPWeak retentivity, for hydrophobic compoundsL13, 5104508.5Yes★★★★2 - 7.5
Inertsil ODS-PHigh steric selectivityL13, 51045029None★★★2 - 7.5
Inertsil ODS-EPA polar functional group embeddedL15104509None★★★★2 - 7.5
Inertsil WP300 C18Analysis of high moleculesL15301509Yes★★★★2 - 7.5
Inertsil ODS-2Ultra pure silica gel is usedL151532018.5Yes★★★★2 - 7.5
Inertsil ODSInertness 1st generationL15,101035014Yes★★2 - 7.5
InertSustain C8First choice with ultra high inertness and high durabilityL72, 3, 5103508Yes★★★★★1 - 10
InertSustainSwift C8High inertness and high durability C8 columnL71.9, 3, 5202006Yes★★★★★1 - 10
Inertsil C8-4Ultra high inertness, High plate count, Low retentivityL72, 3, 5104505Yes★★★★★2 - 7.5
Inertsil C8-3Strong retentivity, Lower column backpressure, Very inertL72, 3, 5, 10104509Yes★★★★2 - 7.5
Inertsil C8Ultra pure silica gel is usedL751532010.5Yes★★2 - 7.5
Inertsil C4Low retentivityL265153207.5Yes★★★★2 - 7.5
Inertsil WP300 C8Suitable for high moleculesL75301504Yes★★★★2 - 7.5
Inertsil WP300 C4 L265301503 ★★★★2 - 7.5
InertSustain PFPExtremely Strong retention of highly polar basic compounds.L433, 51035010Yes★★★★★2 - 7.5
InertSustain PhenylhexylStrong π-π interactions and hydrophobic interactionsL113, 5103509Yes★★★★★1 - 10
InertSustain PhenyExtremely strong π-π interactionsL112, 3, 51035010 ★★★★2 - 7.5
Inertsil Ph-3Strong π-π interactionsL112, 3, 5104509.5 ★★★2 - 7.5
Inertsil PhHigh inertness, Weak π-π interactionsL1151532010Yes★★★2 - 7.5
InertSustain CyanoUltra inertness and can be used in reversed phase modeL103, 5103508Yes★★★★2 - 7.5
 
 

Comparison of Phases

The figure below shows the strength of the stereoselectivity and the pi-interaction with respect to the holding strength of the reversed-phase column. The retention strength, stereoselection system, and strength of pi-interaction are based on the retention coefficient of amylbenzene, retention ratio of o-terphenyl and triphenylene, and retention ratio of amylbenzene and triphenylene, respectively. These are plotted below. In addition, the polar group-encapsulating (embed) type column provides a large effect on other interactions; thus, it was plotted in the type in order to distinguish it from the other columns.

 
Comparison of stereoselectivity between GL Sciences reversed phase columns
Strength of pi electron interaction between GL Sciences reversed phase columns
 

Selectivity Comparison of Reversed Phase Columns with Sample analytes

Selectivity of reversed-phase columns are compared under the same conditions, packing material size and column size. Chemical structural formulas used for the comparison are shown at right. Separation differences between basic compounds, acidic compounds, alkyl benzenes and polyaromatic compounds shows the differences of column selectivity. The more silanol groups on the packing material lead the later elution time of caffeine compared with that of phenol. The higher hydrophobicity of the column shows the later elution time of n-Amylbenzene compared with that of n-Butylbenzene. The higher steric selectivity of the column shows the later elution time of Triphenylene compared with that of o-Terphenyl. 

Analytes for selectivity comparison
Set of Spectra for GL Sciences Reverse phase columns
Set of Spectra for GL Sciences Reverse phase columns 2
 

Reversed Phase Column Selection

For Molecular Weight <5,000

Reversed phase column selection for molecular weight less than 5000
 

For Molecular Weight >5,000

Reversed phase column selection for molecular weight more than 5000
 

For Highly Polar Compounds

Reversed phase column selection for highly polar compounds
 

Reversed Phase Columns Resources:​

  • Download GL Sciences 2022 Reversed Phase Columns Catalog — Download & Discover GL Sciences' Reversed Phase Columns
  • HPLC Reversed Phase Columns Specifications — Summary of GL Sciences HPLC Column Specifications.  Learn More
  • HPLC Reversed Phase Column Selection Overview — Separation mode selection for analyte polarity & HPLC column selection for analyte properties.  Learn More
  • HPLC Column Selection By USP  — Recommended GL Sciences HPLC Columns based on USP Code.  Learn More
  • HPLC Column End-fittings — Summary of GL Sciences' end-fitting style & recommended operating pressure.  Learn More
  • How to Select a Guard Column  — Download guide to select the right guard column.  Learn More
  • Choosing Preparative Column  — Download Guide to select the right preparative column.  Learn More
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