Application Notes

Malachite Green (MG) is a blue-green basic pigment that is used as a remedy against aquatic fungal diseases, it is also used as a bactericidal agent for farmed fish. Current research suggests that it may be carcinogenic, therefore use in foods has been banned in the United States, EU, etc. The distribution and sale of foods in which malachite green is detected is banned in Japan. However, since on rare occasions violations of this ban are confirmed in imported foods, etc., tests are widely conducted by inspection organizations. In this study, malachite green and its biotransform Leucomalachite Green (LMG) were extracted from grilled eel extract and purified using a mixed-mode polymer solid phase (InertSep MCX FF, LMG) and then analyzed by LC / MS / MS with a test method complied with ...

When the pH of mobile phase is changed in the analysis of reverse phase mode on HPLC, the dissociation / non-dissociation state changes depending on the compound, so the retention time changes. Normally, the retention time is longer in non-dissociated state and shorter in dissociated state. This time, using Inertsil WP300 C18, we analyzed the basic compound saccharin and the acidic compound phthalic anhydride standard, and found that the elution order changed under acidic and neutral conditions. We also report that it was estimated that phthalic anhydride in the sample was partially changed to phthalic acid.