Sample can be neutralized and maintained in stable condition by putting neutral solution in tube when recovering anti-body.
As shown below, the anti-body concentrations were determined quantitatively from medium of CHO cells. The purified antibodies show very less impurities by the results from electrophoresis.
Both MonoSpin ProA and MonoSpin ProG have 90% recovery (max 0.4 mg MonoSpin Pro A, max 0.3 mg MonoSpin ProG). MonoSpin ProG enables higher recovery and purification of IgG3 over MonoSpin ProA.
Relation between amount of IgG addition and collection of antibodies. MonoSpin ProA (left), MonoSpin ProG (right)
The monolithic silica phase used in MonoSpin columns is state-of-the-art in stationary phase technology allowing high recovery and adsorption and desorption performance. The structure of the silica allows for less obstruction of the binding proteins compared to conventional particle based phases allowing for much higher efficiency of separation.
MonoSpin ProA/ProG enables you to remove proteins such as BSA and Gelatin in anti-body solutions without dilution.
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MonoSpin ProG spin column L Type 4 pcs.