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SPE Columns / Cartridges
Columns with pre-packed sorbents for sample pre-treatment by solid phase extraction. GL Sciences InertSep SPE column sorbents come in two types of sorbents: polymer-based sorbents which have the widest pH compatibility and silica-based sorbents which are more cost-effective and have higher physical strength. Columns are available in various sizes and formats including cartridges, luer-type devices and 96-well plates for compatibility with every SPE method (gravity, vacuum, positive-pressure, to centrifugal systems). Read more
High-Quality Solutions for Efficient Sample Preparation InertSep QuEChERS Kit is a product developed by leveraging decades of expertise in manufacturing high-quality chromatography products. This kit efficiently removes impurities such as organic acids, sugars, fatty acids, pigments, chlorophyll, and carotenoids from samples, enhancin...
From $104.00
InertSep HLB is a solid-phase extraction column that can effectively extract compounds with a wide range of physical properties from non-polar to high polar.
From $173.00
InertSep MAX FF is a sorbent based on styrene divinylbenzene polymer modified by a strong anion-exchange group. Its reversed-phase, anion-exchange action and exceptional retention of acidic compounds make it suitable for recovery from samples containing basic and neutral impurities.
From $219.00
InertSep MCX FF is a sorbent based on styrene divinylbenzene polymer modified by a strong cation-exchange group. Its reversed-phase, cation-exchange action and exceptional retention of basic compounds make it suitable for recovery from samples containing acidic and neutral impurities.
From $219.00
InertSep WAX FF is a sorbent based on styrene divinylbenzene polymer modified by a weak anion-exchange group. Its reversed-phase, anion-exchange action and exceptional retention of acidic compounds make it suitable for purification of samples containing basic and neutral impurities. By controlling the pH, it can be used to recover acidic ...
From $219.00
InertSep WCX FF is a sorbent based on styrene divinylbenzene polymer modified by a weak cation-exchange group. Its reversed-phase, cation-exchange action make it ideal for pretreatment of basic compounds such as MCX. By controlling the pH, it can be used to recover strongly basic compounds under acidic conditions.
From $219.00
InertSep C18 is a silica-based sorbent modified with C18 (octadecyl) functional groups being utilized for non-polar interaction. With our high-level end-capping technology, cationic interaction with the silanol groups on the gel is virtually eliminated, which reduces adsorption of basic compounds. This sorbent is suitable for removing lip...
From $155.00
InertSep C18-B is a silica-based sorbent modified with C18 (octadecyl) functional groups being utilized for non-polar interaction. In addition to the interaction, secondary interaction can be expected on this mono functional, general purpose sorbent.
From $155.00
InertSep C18 FF is an arranged version of InertSep C18 for applications that need high flow late. This sorbent is also good at viscous biological sample and large volume sample to expedite the procedure.
From $155.00
InertSep C18-C is a silica-based sorbent modified with C18 (octadecyl) functional groups being utilized for non-polar interaction. In addition to the general purpose non-polar interaction, secondary interaction between unbonded silanol groups on silica substrate and isolates can be expected on this trifunctional C18.
From $155.00
InertSep C18 ENV is a C18 solid phase synthesized for water analysis. It is useful for sample treatment of anion surfactants in water.
From $164.00
InertSep C18-C FF is an arranged version of InertSep C18-C for applications that need high flow late. This sorbent is also good at viscous biological sample and large volume sample to expedite the procedure.
From $155.00
InertSep C8 is a silica-based sorbent modified with C8 (octyl) function groups that offers a weaker non-polar interaction than C18. InertSep C8 is used for isolates that are strongly retained too much on C18. With high-level end-capping, cationic interaction with the silanol groups on silica substrate is virtually eliminated and adsorptio...
From $155.00
InertSep C2 is a silica-based sorbent modified with C2 (ethyl) functional groups being utilized for a weaker non-polar interaction than C8. InertSep C2 is used for isolates that are strongly retained too much on C8. As end-capping is accomplished on this sorbent, cation exchange interaction with none bonded silanol groups is virtually eli...
From $146.00
InertSep CH is a silica-based sorbent modified with cyclohexyl functional groups that gives this sorbent a similar moderate polarity with InertSep C2. InertSep CH offers a unique selectivity for the extraction of certain chemical compounds, compared with C18, C8, C2 and PH.
From $219.00
InertSep PH is a silica-based sorbent modified with phenyl functional groups having a similar non-polar interaction to C8. Phenyl functional group has π-π bond interaction which allows an isolate molecule bearing benzene ring, such as aromatic compounds to selectively interact with phenyl functional group on the sorbent.
From $155.00
InertSep PLS-2 is a SDB (Styrene divinylbenzene) polymer based reversed phase mode sorbent. Compared to a silica based C18 sorbent, InertSep PLS-2 has a quite higher sorbent retentive capacity and better stability in pH range.
From $182.00
InertSep PLS-3 is a copolymer based sorbent comprised of nitrogen-containing methacrylate and SDB, it exhibits a wide enough range of retention capability from highly polar to hydrophobic compounds.
From $200.00
InertSep RP-1 is a copolymer based sorbent comprised of methacrylate and SDB, it exhibits a wide enough range of retention capability from low-polar to hydrophobic compounds. It is also suit for clean up.
From $270.00
InertSep C18-B FF is an arranged version of InertSep C18-B for applications that need high flow late. This sorbent is also good at viscous biological sample and large volume sample to expedite the procedure.
InertSep RP-2 is a SDB polymer based sorbent modified with weak anion exchange and hydrogen bonding functional groups. This sorbent is suit for concentration of low-retentive polar compounds with RP-1 and simultaneous screening under polar interactions.
InertSep RP-C18 is a SDB polymer based sorbent modified with alkyl chain.
From $250.00
InertSep MPC is a SDB polymer based sorbent modified with strong cation exchange and C18 functional groups. Because of its hydrophobic and ion-exchange properties, it is useful for pretreatment of basic compounds from hydrophobic compounds in blood and urine for simultaneous analysis of metabolites. It can also be used for separation and...
From $240.00
InertSep Pharma is a copolymer based sorbent comprised of nitrogen-containing methacrylate and SDB. This sorbent was developed for simultaneous screening of drug metabolites in biological sample.
From $246.00
InertSep Pharma FF is an arranged version of InertSep Pharma for applications that need high flow rate and good at viscous biological sample and large volume sample.
From $291.00
InertSep MA-1 is a methacrylate polymer based sorbent modified with strong anion exchange functional groups. The primary advantages of this sorbent are high hydrophilicity and attainability of easy and effective elution of isolates.
From $273.00
InertSep MA-2 is a methacrylate polymer based sorbent modified with, which includes a weak anion exchange function group. Because polymer base gel occurs little secondary interaction, SPE by genuine anion exchange interaction can be achieved with this sorbent.
From $273.00
InertSep MC-1 is a methacrylate polymer based sorbent modified with strong cation exchange functional groups. The primary advantages of this sorbent are high hydrophilicity and attainability of easy and effective isolation of cationic compounds.
From $273.00
InertSep MC-2 is a methacrylate polymer based sorbent modified with week cation exchange functional groups. This sorbent is suitable for SPE of strong anion compounds in ion exchange mode. Because polymer base gel occurs little secondary interaction, SPE by genuine cation exchange interaction can be achieved with this sorbent.
From $273.00
InertSep ME-1 is a methacrylate copolymer based solid sorbent modified with iminodiacetic acid, weak cation exchange functional groups. It is highly hydrophilic and does not retain monovalent Na ion or K ion, but it does retain metal divalent or more cations. This offers selective concentration of such metal ions and is suitable for custo...
From $282.00
InertSep ME-2 is a chelating resin sorbent, developed for SPE of trace metal ions in seawater. As this sorbent does not retain Ca and Mg ions, desalting can be achieved by passing the sample through the sorbent and wash with purified water.
From $282.00
InertSep CN is a silica-based sorbent modified with cyanopropyl functional groups. Having both non-polar and polar interactions, InertSep CN is ideal for applications in which extremely non-polar isolates would irreversibly retain on more non-polar sorbents such as C18 or C8 and very polar isolates might be retained irreversibly on the mo...
From $219.00
InertSep 2OH is a silica-based sorbent modified with diol functional groups. Being a fairly polar sorbent, InertSep 2OH is typically used for polar extractions from low-polar or non-polar solvents.
From $219.00
InertSep SI is a bare silica, which has strong polar interaction. It offers selective separation for similar compounds in structure using low-polar solvents. InertSep SI is regarded as the most polar sorbent available. Care should be taken with humidity and polar solvents, when using this material.
From $164.00
InertSep SI FF is an arranged version of InertSep SI for applications that need high flow late. This sorbent is also good at viscous sample and large volume sample to expedite the procedure.
From $164.00
InertSep AL-A is packed with alumina (Al2O3). Available in Al2O3 acidic format.
From $182.00
InertSep AL-B is packed with alumina (Al2O3). Available in Al2O3 basic format.
From $182.00
InertSep AL-N is packed with alumina (Al2O3). Available in Al2O3 neutral format.
From $182.00
Florisil SPE cartridges are packed with magnesium silica, highly polar material which strongly adsorb polar compounds from none polar matrices and typically used for sample cleanup of organic extracts.
From $155.00
InertSep FL-PR cartridges are packed with FL-PR, which is used for sample cleanup for analysis of residual pesticides in crops. This sorbent is also good at viscous sample and large volume sample to expedite the procedure.
From $155.00
InertSep CBA is a silica-based sorbent modified with carboxyl ethyl functional groups. The primary interactions of this sorbent are cation exchange and secondary interactions are week-polar and non-polar. This sorbent is suitable for extraction of drugs with strongly cationic amine groups.
From $191.00
InertSep PRS is a silica-based sorbent modified with propyl-sulfonyl functional groups. The primary interactions of this sorbent are anion exchange and secondary interactions are slightly non-polar.
From $200.00
InertSep SCX is a silica-based sorbent modified with benzene sulfonyl propyl functional groups. The primary interaction is a combination of both non-polar and strong cation exchange. Because the non-polar interactions on InertSep SCX is stronger than those of InertSep PRS, it is suitable for such an extraction that the combination of non-...
From $219.00
InertSep SCX-2 employs the same chemical modification with InertSep SCX. The only difference is that benzene sulfonyl propyl functional groups are bonded more densely on the silica surface to increase ion exchange capacity and retentivity.
From $219.00
InertSep PCB is a two layer SPE cartridge packed with SCX and SI. It has been designed for the extraction of PCBs from complex matrix. InertSep PCB is generally used for the extraction of isolates from matrices such as oil and milk.
From $364.00
InertSep NH2 is a silica-based sorbent modified with an amino-propyl functional groups. Anion exchange and polar interaction are combined as a primary interactions. As secondary interactions, it has weak non-polar interactions. Similar to InertSep 2OH and InertSep SI used in normal phase mode, InertSep NH2 can be used for the separation o...
From $228.00
InertSep NH2 FF is an arranged version of InertSep NH2 for applications that need high flow late. This sorbent is also good at viscous sample and large volume sample to expedite the procedure.
From $200.00
InertSep PSA is a silica-based sorbent modified with an ethylene-diamine-N-propyl functional groups. The primary interactions of this sorbent are anion exchange and secondary interactions are week non-polar.
From $228.00
Cartridge
- Cartridge volume: 1, 3, 6, 12, 20, 60, 150 mL
- Cartridge material: PP housing and PE frit
- Purpose: Processing of small liquid samples up to bulk
96-well plate
- Cartridge volume: 1, 2 mL
- Cartridge material: PP housing and PE frit
- Purpose: Rapid processing for multiple samples
Luer Device type
Slim & Slim-J type
- Dimensions: Slim: 8.8 mm O.D. length 32 mm, 21mm; Slim-J: 8.8 mm O.D. length 51 mm, 31 mm
- Cartridge material: PP housing and PE frit
- Purpose: Tandem processing and automatic SPE system
Mini type
- Dimensions: 12.7 mm O.D. length 32 mm, 21 mm
- Cartridge material: PP housing and PE frit
- Purpose: manual processing, tandem processing and automatic SPE system
Concept of Solid Phase Extraction (SPE)
Main approaches of SPE
The principle of SPE is divided into the following two methods:
1. Retention of Target Analyte
2. Retention of Sample Matrix
General Steps of SPE
General Methods for Sample Processing
Solid phase extraction methods include: gravity-based, positive pressure, vacuum, and centrifugal separation systems.
Available Sorbents for SPE Columns
InertSep SPE cartridges come in two major types of base gels - Polymer-based sorbents and Silica-based sorbents. Specialty phases are also available.
Polymer-based Sorbents
The advantages of polymer-based sorbent are their availability in a wide pH range and the absence of secondary interaction which can occur with silica-based sorbents.
Silica-based Sorbents
Silica-based sorbent materials are more cost-effective and have higher physical strength compared with polymer-based sorbent materials. Silica offers a wide variety of separation mechanisms using a combination of primary functional group interaction with secondary interactions due to the nature of silica.
Specialty Phases
Retentive Capacity of a Sorbent Compared to Sorbent Mass
Bed volume is the quantity of the solvent necessary to replace the air trapped in the solid phase. Void volume is equivalent to the bed volume.
How to Select a Sorbent
One of the most important elements to achieve successful solid phase extraction is selection of a sorbent which should be suitable for both the sample matrix and the target analyte. The sorbent should be carefully selected, taking into account the chemical properties of the target analyte, such as its solvent solubility and the physical nature of the sample matrix. In addition, it is important to develop conditions that are optimal for retaining the target analyte, while removing the sample matrix, then selecting an elution solvent for maximum recovery of the target analyte.
Recommendation for Selecting an Ion Exchange Sorbent
In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.
Recommendation for Selecting an Ion Exchange Sorbent
InertSep Quality Control
InertSep SPE columns are manufactured under strict quality control at our Fukushima factory which has obtained International Organization for Standardization ISO-9001 certification.
SPE Columns / Cartridges Resources:
- Download our entire 2022 Consumables Catalog — Download
- Search Application Notes For SPE Columns / Cartridges — Search Applications Database
- SPE Column / Cartridge selection guide — Download the guide to select the right sorbent and column / cartridge Download
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