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SPE Columns / Cartridges
Columns with pre-packed sorbents for sample pre-treatment by solid phase extraction. GL Sciences InertSep SPE column sorbents come in two types of sorbents: polymer-based sorbents which have the widest pH compatibility and silica-based sorbents which are more cost-effective and have higher physical strength. Columns are available in various sizes and formats including cartridges, luer-type devices and 96-well plates for compatibility with every SPE method (gravity, vacuum, positive-pressure, to centrifugal systems). Read more
Cartridge
- Cartridge volume: 1, 3, 6, 12, 20, 60, 150 mL
- Cartridge material: PP housing and PE frit
- Purpose: Processing of small liquid samples up to bulk
96-well plate
- Cartridge volume: 1, 2 mL
- Cartridge material: PP housing and PE frit
- Purpose: Rapid processing for multiple samples
Luer Device type
Slim & Slim-J type
- Dimensions: Slim: 8.8 mm O.D. length 32 mm, 21mm; Slim-J: 8.8 mm O.D. length 51 mm, 31 mm
- Cartridge material: PP housing and PE frit
- Purpose: Tandem processing and automatic SPE system
Mini type
- Dimensions: 12.7 mm O.D. length 32 mm, 21 mm
- Cartridge material: PP housing and PE frit
- Purpose: manual processing, tandem processing and automatic SPE system
Concept of Solid Phase Extraction (SPE)
Main approaches of SPE
The principle of SPE is divided into the following two methods:
1. Retention of Target Analyte
2. Retention of Sample Matrix
General Steps of SPE
General Methods for Sample Processing
Solid phase extraction methods include: gravity-based, positive pressure, vacuum, and centrifugal separation systems.
Available Sorbents for SPE Columns
InertSep SPE cartridges come in two major types of base gels - Polymer-based sorbents and Silica-based sorbents. Specialty phases are also available.
Polymer-based Sorbents
The advantages of polymer-based sorbent are their availability in a wide pH range and the absence of secondary interaction which can occur with silica-based sorbents.
Silica-based Sorbents
Silica-based sorbent materials are more cost-effective and have higher physical strength compared with polymer-based sorbent materials. Silica offers a wide variety of separation mechanisms using a combination of primary functional group interaction with secondary interactions due to the nature of silica.
Specialty Phases
Retentive Capacity of a Sorbent Compared to Sorbent Mass
Bed volume is the quantity of the solvent necessary to replace the air trapped in the solid phase. Void volume is equivalent to the bed volume.
How to Select a Sorbent
One of the most important elements to achieve successful solid phase extraction is selection of a sorbent which should be suitable for both the sample matrix and the target analyte. The sorbent should be carefully selected, taking into account the chemical properties of the target analyte, such as its solvent solubility and the physical nature of the sample matrix. In addition, it is important to develop conditions that are optimal for retaining the target analyte, while removing the sample matrix, then selecting an elution solvent for maximum recovery of the target analyte.
Recommendation for Selecting an Ion Exchange Sorbent
In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.
Recommendation for Selecting an Ion Exchange Sorbent
InertSep Quality Control
InertSep SPE columns are manufactured under strict quality control at our Fukushima factory which has obtained International Organization for Standardization ISO-9001 certification.
SPE Columns / Cartridges Resources:
- Download our entire 2022 Consumables Catalog — Download
- Search Application Notes For SPE Columns / Cartridges — Search Applications Database
- SPE Column / Cartridge selection guide — Download the guide to select the right sorbent and column / cartridge Download