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InertSustain AX-C18 HPLC Column, 3 µm, 100 x 4.6 mm

SKU: 5020-91050
$600.00
+ -

Specifications

InertSustain AX-C18 HPLC Column, 3 µm, 100 x 4.6 mm SKU: 5020-91050

Specifications
Attribute nameAttribute value
Column or GuardColumn
Column formatAnalytical
PhaseInertSustain AX-C18
Base MaterialHigh Purity ES Silica Gel
Functional groupOctadecyl + Alkylamino (tertiary amine)
Particle size3 µm
Length100 mm
Inner diameter4.6 mm
ModeReversed phase, Ion exchange
Surface area200 m2/g
Pore size200 Å (20 nm)
Pore volume1.00 mL/g
End cappingYes
Carbon loading8.0%
pH Range1-9, 1-9
Unit size1 pc.
Max Operating Pressure20 MPa(200 bar)
USP CodeL1, L78

InertSustain AX-C18 - Overview

Retention behavior that combines both reversed phase and anion exchange

 

InertSustain AX-C18 Anion Exchange Column has increased retention of acidic compounds/decreased retention of basic compounds compared to typical C18 columns.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of nucleobases and monophosphates graph

Compounds:

1. Cytosine (basic)  2. Uracil (neutral)  3. CMP (acidic)  4. UMP (acidic)  5. Adenine (basic)  6. AMP (acidic)

InertSustain AX-C18 Anion Exchange Column HPLC Columns compounds 1
InertSustain AX-C18 Anion Exchange Column HPLC Columns compounds 2
 

Excellent retention for acidic compounds

 

No ion pair reagent in the eluent means highly polar acidic compounds are retained.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of sugar-nucleotides

Strongly retains highly polar acidic compounds which normally cannot be retained by C18 columns for analysis of highly polar compounds!

InertSustain AX-C18 Anion Exchange Column strongly retains acidic compounds even if the salt concentration of the eluent is low. Also compatible with MS analysis.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of monophosphoric acid

Strongly retains highly polar acidic compounds even when salt concentration is as low as 10 mM! Sharp elution peaks!

Can be analyzed at low salt concentration. Compatibility with MS!

 

Excellent reproducibility and quality

 

Reproducibility issues with mixed mode are solved by GL Sciences' original packing and manufacturing technology.

InertSustain AX-C18 Anion Exchange Column HPLC Columns lot to lot variation graph
InertSustain AX-C18 Anion Exchange Column HPLC Columns lot to lot variation in acidic compounds graph

Excellent reproducibility of retention time in mixed‐mode columns where reproducibility is difficult to control!

Applications

There are application notes for InertSustain AX-C18 HPLC Column, 3 µm, 100 x 4.6 mm.

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InertSustain AX-C18 HPLC Column, 3 µm, 100 x 4.6 mm