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Inertsil Hybrid-C18

Inertsil Hybrid-C18
Inertsil Hybrid-C18

High-pH Tolerant Core-Shell Column for Robust and Reproducible Performance

Inertsil Hybrid-C18 is a silica-based reversed-phase column engineered with hybrid organic–inorganic bonding technology, providing exceptional chemical stability across the full pH range (1–12). Features • pH range 1–12 for both acidic and basic conditions • Excellent batch-to-batch reproducibility • Compatible with LC/MS, LC/MS/MS, and high-pressure LC systems • Long lifetime and high durability, even under basic pH • USP L1 classification (C18)
Specifications
  • Base Material: High-pH Stable Hybrid Silica
  • Particle size: 1.7 μm, 3.5 μm, 5 µm
  • Carbon loading: 15.0%
  • End capping: Yes
  • Pore size: 165 Å (16.5 nm)
  • USP Code: L1
  • Pore volume: 1.00 mL/g
  • Max Temperature: 80°C (pH 1 - 10), 60°C (pH 1 - 12)
  • Surface area: 185 m²/g
  • pH Range: 1-12
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Inertsil Hybrid-C18

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GL Sciences: Trusted by Scientists Since 1968

About Inertsil Hybrid-C18

 

A Legacy of Quality

 

Since the launch of Inertsil ODS-3 in 1994, we have consistently manufactured HPLC columns in-house—from the synthesis of base silica to final packing. This fully integrated production process is the foundation of our ability to deliver high-quality, highly reliable columns with unmatched consistency. 

Inertsil Hybrid-C18 was newly developed to meet the evolving needs of users, offering exceptional durability under high-pH mobile phase conditions. This column expands application flexibility while maintaining the trusted performance of the Inertsil series.

 
 
 

One Column, Infinite Possibilities

Impact of Mobile Phase pH on Selectivity

 

These chromatograms demonstrate how changes in mobile phase pH influence the selectivity and resolution of analytes. Inertsil Hybrid-C18 of hybrid silica gel allows stable performance across this wide pH range without loss of efficiency and reproducibility

 
 

  • Basic compounds (pKa ≈ 9) show stronger retention at high pH due to reduced ionization

  • Acidic compounds (e.g., COOH) retain better at low pH where ionization is suppressed.

  • Understanding pKa enables control over separation behavior.

 
 
 
 

Alkali-Resistant Hybrid Silica Technology

 

Superior Durability Under High pH Conditions (pH 11.5)

 

With its advanced hybrid silica technology, Inertsil Hybrid-C18 delivers exceptional resistance to alkaline mobile phase condition. Even under high-pH mobile phase conditions that typically cause dissolution of conventional packings, the column maintains its structural integrity.

This unique robustness prevents loss of theoretical plates, guaranteeing reliable peak resolution, reproducible separations, and extended column lifetime.

 

Mobile Phase for Durability Testing

Column : 5 µm, 150 × 2.1 mm I.D.
Eluent : A) 50 mM Phosphate Buffer (pH 11.5) in H2O
    B) CH3CN
A/B = 90/10, v/v
Flow Rate : 0.2 mL/min
Col. Temp. : 40 °C

Mobile Phase for Analysis

Eluent : A) H2O
    B) CH3CN
A/B = 35/65, v/v
Flow Rate : 0.2 mL/min
Col. Temp. : 40 °C
Detection : 254 nm
Sample : Naphthalene

 
 

Consistent Theoretical Plate Count at High pH

Key features visual
 
 

Excellent batch-to-batch reproducibility

Comparison of three different production lots of Inertsil Hybrid-C18 show virtually identical retention and peak shape, demonstrating outstanding lot-to-lot consistency. This high reproducibility ensures method robustness and eliminates the need for revalidation when switching lots.

 
 
 

Superior Performance Across Challenging Analytes with Inertsil Hybrid-C18

Inertsil Hybrid-C18 demonstrates excellent peak shape for basic compounds, acidic compounds, and metal-coordinating compounds, outperforming competitor columns.This high-level performance is achieved through our proprietary hybrid silica technology, which offers superior resistance to peak tailing and adsorption issues under a wide range of pH conditions.

 
 
 

Column Hardware Lineup

 

Wide Range of Dimensions and Hardware Options

 

Hardware is available in two choices: stainless steel (SS) and biocompatible PEEK, making it suitable for everything from routine analyses to LC/MS, LC/MS/ MS, and sensitive biological applications.

Customized Dimensions Available on Request

In addition to our standard lineup, custom column dimensions and configurations can be made to order, including preparative-scale dimensions and specialty hardware options.

 
 
Key features visual
 
 

Applications

 
 

Guanine-based antiviral agent

 
Chromatogram - Guanine-based antiviral agent
 

Conditions
Column : 5 µm, 150 × 2.1 mm I.D.
Eluent : 10 mM HCOONH4 in H2O
Flow Rate : 0.3 mL/min
Col. Temp. : 40 °C
Detection : UV 254 nm
Inj. Vol. : 1 µL
Analytes : 1. Ganciclovir  2. Aciclovir  3. Penciclovir (100 mg/L each)

 
 

Nucleoside-based antiviral drug

 
Chromatogram - Nucleoside-based antiviral drug
 

Conditions
Column : 3.5 µm, 150 × 2.1 mm I.D.
Eluent : 10 mM HCOONH4 in H2O
Flow Rate : 0.3 mL/min
Col. Temp. : 40 °C
Detection : UV 240 nm
Inj. Vol. : 1 µL
Analytes : 1. Ribavirin  2. Cytarabine  3. Gemcitabine  4. Lamivudine  5. Vidarabine (100 mg/L each)

 
 

Azithromycin Tablet of Assay under USP condition

 
Chromatogram — Azithromycin Tablet Assay (USP)
 

Conditions
Column : 5 µm, 250 × 4.6 mm I.D.
Eluent : CH3CN/Buffer* = 65/35, v/v
Flow Rate : 2.0 mL/min
Col. Temp. : 50 °C
Detection : UV 210 nm
Inj. Vol. : 100 µL
Analyte : Reference Standard in Eluent — 1. Azithromycin dihydrate 1 mg/mL

*Dissolve 4.6 g of monobasic potassium phosphate anhydrous in 900 mL of water. Adjust with 1 N sodium hydroxide to pH 7.5, and dilute with water to 1 L.

 
 

Azithromycin Tablet of Dissolution under USP condition

 
Chromatogram — Azithromycin Tablet Dissolution (USP)
 

Conditions
Column : 5 µm, 150 × 4.6 mm I.D.
Eluent : CH3CN/CH3OH/Solution* = 9/3/8, v/v/v
Flow Rate : 1.5 mL/min
Col. Temp. : 50 °C
Detection : UV 210 nm
Inj. Vol. : 50 µL
Sample : Reference Standard — 1. Azithromycin dihydrate 0.25 mg/mL

*4.4 mg/mL of dibasic potassium phosphate and 0.5 mg/mL of sodium 1-octanesulfonate; adjusted with phosphoric acid to a pH of 8.20 ± 0.05.

 
 

Antihistamine

 
Chromatogram — Antihistamine
 

Conditions
Column : 3.5 µm, 150 × 2.1 mm I.D.
Eluent : A) 10 mM HCOONH4 in H2O   B) CH3CN
Program : A/B = 80/20 – 15 min – 20/80 – 0.1 min – 80/20, v/v
Flow Rate : 0.3 mL/min
Col. Temp. : 40 °C
Detection : UV 240 nm
Inj. Vol. : 2 µL
Analytes : 1. Epinastine  2. Chlorpheniramine  3. Fexofenadine  4. Triprolidine  5. Ketotifen  7. Azelastine  8. Hydroxyzine  9. Homochlorcyclizine  10. Clemastine  11. Loratadine
(50 mg/L each)

 
 

Antidepressant

 
Chromatogram — Antidepressant
 

Conditions
Column : 3.5 µm, 150 × 2.1 mm I.D.
Eluent : A) 10 mM HCOONH4 in H2O   B) CH3CN
Mix : A/B = 50/50, v/v
Flow Rate : 0.3 mL/min
Col. Temp. : 40 °C
Detection : UV 230 nm
Inj. Vol. : 1 µL
Analytes : 1. Nortriptyline  2. Imipramine  3. Amitriptyline  4. Sertraline  5. Clomipramine  6. Mianseline
(100 mg/L each)

 
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