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HPLC Columns
GL Sciences columns provide excellent separation and resolution of target compounds featuring new high-purity evolved surface silica. GL Sciences columns silica base is uniquely modified to provide maximum column performance including low operating back-pressure, high inertness, high efficiency and wide solvent compatibility. Get the appropriate column from a large selection of column configurations and formats compatible with many HPLC methods including analytical, preparative, capillary HPLC and UHPLC. Read more
Inertsil Ph has phenethyl groups bonded to silica gel which offers weak pi-pi interactions. As it is modified with phenethyl groups, hydrophobic interactions between alkyl chain and analytes play an important role in separation as well as π-π interactions. To change the selectivity or elution pattern drastically, InertSustain Phenyl is re...
From $255.00
In general, the stability and reproducibility of the Cyano phase available in the market are poor. Many batch-to-batch or lot-to-lot reproducibility issues are occurring at many laboratories. The InertSustain Cyano columns were developed to resolve these problems and are designed using the most modern LC column technology available provid...
From $191.00
InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phase...
From $191.00
Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime. ...
From $191.00
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when...
From $191.00
Modified with amide groups, the ProteoSil HILIC column excels in the separation of highly hydrophilic compounds, peptides, glycans, and oligonucleotides.
From $246.00
InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of ...
From $191.00
Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous ana...
From $191.00
Inertsil Diol column has characteristics of dihydroxypropyl group bonded phase. It shows unique selectivity normal phase mode. The separation mechanism of diol column is featured by hydrogen bonding interactions between diol groups and polar compounds. Diol columns provide an alternative selectivity to silica columns often with increased ...
From $191.00
Equipped with dihydroxypropyl groups bonded to silica gel, the ProteoSil 300-SEC column is suited for the analysis of large molecules and can be used as a dial column.
From $246.00
Constructed for aqueous size-exclusion chromatography, the ProteoSil 100-SEC can handle higher operating pressures and is suitable for series column configurations.
From $246.00
Inertsil SIL-100A is a pure silica gel column available in normal phase mode. Because of the high quality of its silica gel, Inertsil SIL-100A achievs separation with sharp peaks and provides high column-to-column reproducibility. This excellent silica gel ideally designed for HPLC is the basis for “Inertsil 3-series” of GL Scineces. GL S...
From $191.00
Inertsil CN-3, Cyanopropryl groups bonded to Silica gel with high density. And it is not only increase difference recognition of hydrophilicity, but also increase the durability. It is difficult to increase the both performance with other previous cyanopropryl columns. Inertsil CN-3 can be cleaned with 100 % aqueous solvent because non-sp...
From $191.00
Inertsil SIL-150A is ultra pure silica gel column, and this ultra pure silica gel contains very low level of metal impurities and is durable and free from dents and cracks which can cause premature column failure. Compared to Inertsil SIL-100A, the silica’s surface area is smaller (320 m²/ g). Retentivity of Inertsil SIL-150A is weaker th...
From $255.00
Inertsil WP300 SIL is pure silica gel phase with wide pores(300 Å). It is available for analysing compounds including large molecules. As the pore becomes wider, the surface area of silica gel is smaller. Since the interactions between the analyte and silica gel occur on the sIlica surface, smaller surface area means less interactions and...
From $191.00
Inertsil WP300 Diol has dihydroxypropyl group bonded to silica gel with pore size 300 Å and is capable of analyzing large molecules. Like Inertsil Diol, Inertsil WP300 Diol can be used for both aqueous SEC (Size Exclusion Chromatography) and organic SEC. Also, as a diol column, Inertsil WP300 Diol can be used in both normal phase and reve...
From $191.00
Inertsil AX has diethylamino groups bonded to silica gel by an alkyl chain. The diethylamino groups offer anionic functions required for anion exchange chromatography. It is mainly used for analyses of acidic compounds. Conventional ion-exchange columns used to show inconsistent results from lot to lot. However, Inertsil AX is manufacture...
From $191.00
The Inertsil CX Cation Exchange HPLC Column has benzenesulfonyl groups bonded to silica gel by an alkyl chain. The sulfonyl groups at the end of the structure offer cationic functions required for the cation exchange chromatography. It is mainly used for analyses of basic compounds. Inertsil CX is manufactured under strict quality control...
From $191.00
Specifications • Base Material : 3 Series High Purity Silica Gel • Particle Size : 3 μm, 5 μm • Surface Area : 450 m²/g • Pore Size : 100 Å (10 nm) • Pore Volume : 1.05 mL/g • Functional Group : Octadecyl • End-capping : Yes • Carbon Loading : 15 % • USP Code : L1
From $273.00
Workable at a broad range of linear velocity from 0.5 to 5 mm/s without sacrificing efficiency and separation at high speed. The number of theoretical plates produced by MonoCap C18 Fast-flow is nearly equivalent to a totally porous particle type capillary column packed with a 5µm packing material. Columns are protected by either metal or...
From $1,046.00
Maximizing all the benefits and advantages of monolithic technology, MonoCap High Resolution and High Resolution Ultra are appropriate for the efficient separation of peptides and protein digests. MonoCap High Resolution provide extremely high efficiency, delivering over 200,000 plates for a 2,000 mm length column. The High Resolution...
From $4,646.00
MonoCap C18 Nano-flow produces higher number of theoretical plates compared to a totally porous particle type capillary column packed with a 3µm packing material. It can be operated at a wide range of flow rate with low back pressure and achieve very high sensitive results in Nano-LC-ESI/MS applications. Columns are protected by either me...
From $1,100.00
MonoCap Trap columns have a relatively big throughpore and workable at a high flow rate such as 10 μL/min. This benefit makes MonoCap Trap columns to be appropriate for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection. End-fittings are 1/16“ (10-32 UNF). 1/32“...
From $219.00
MonoSelect C18 for HTS is based on monolithic silica technology. The very low flow resistance and the excellent separation performances make it suitable for High-Throughput Screenings (HTS). Due to its metal-free hardware, sharp peaks can be obtained also when analyzing metal chelators. The particular structure of MonoSelect C18 for HTS p...
From $237.00
MonoSelect nPEC uses nanoparticle exclusion chromatography (nPEC). It can rapidly separate nano-particles and free-low molecule compounds. Specifications • Silica:High Purity Monolithic Silica Gel • Through Pore Size: 1 µm • Surface Area: 340 m2／g • Pore Size (Moso-pore Size): 110 Å (11 nm) • Functional Group: Hydrophilic Pol...
From $1,000.00
MonoSelect RP-mAb is an HPLC column specialized for monoclonal antibody analysis. Monolithic silica delivers “Strong Retentivity and Low Adsorption”. Sharp peaks can perform even in large molecular protein analysis such like antibodies. Specifications • Base Material: Highly Pure Monolithic Silica • Through Pore Size: 1 µm • Mos...
From $264.00
SKU# 5020-90005
Delay Column for PFAS is packed with high-purity activated carbon, which is able to retain contaminants originating from the mobile phase or the LC system. Elution of retained contaminants is delayed allowing for the effects of the contaminants on the analysis to be reduced. This column is quite useful for PFAS (Per- and Polyfluoroalkyl S...
$764.00
Non-polar phases including alkyl phases such as C18, C8, C4 and other functionalities such as phenyl and cyano groups. GL Sciences provides a diverse range of reverse phases for different analyte retentivities and selectivities by group modification. A high degree of analyte targeting can be achieved using our selection of reverse phases.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| InertSustain C18 | First choice with ultra high inertness and high durability | L1 | 2, 3, 5, 10 | 10 | 350 |
| InertSustain AQ-C18 | First choice for high polar compounds | L1, L96 | 1.9, 3, 5 | 10 | 350 |
| InertSustainSwift C18 | First analysis with ultra high inertness and high durability | L1 | 1.9, 3, 5 | 20 | 200 |
| InertSustain AX-C18 | Analysis of anionic highly polar compounds | L1, L78 | 3, 5 | 20 | 200 |
| InertCore Plus C18 | Ideal for analyses requiring a high number of theoretical stages | L1 | 2.6 | 9 | 200 |
| Inertsil ODS-HL | Ultra high retentivity, High-density bonding of C18 phase | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-4 | Ultra high inertness, High plate count, Medium retentivity | L1 | 2, 3, 5 | 10 | 450 |
| Inertsil ODS-4V | Inertsil ODS-4 Validated column | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-3 | Strong retentivity, Lower column backpressure, Very inert | L1 | 2, 3, 4, 5, 10 | 10 | 450 |
| Inertsil ODS-3V | Inertsil ODS-3 Validated column | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-SP | Weak retentivity, for hydrophobic compounds | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-P | High steric selectivity | L1 | 3, 5 | 10 | 450 |
| Inertsil ODS-EP | A polar functional group embedded | L1 | 5 | 10 | 450 |
| Inertsil WP300 C18 | Analysis of high molecules | L1 | 5 | 30 | 150 |
| Inertsil ODS-2 | Ultra pure silica gel is used | L1 | 5 | 15 | 320 |
| Inertsil ODS | Inertness 1st generation | L1 | 5, 10 | 10 | 350 |
| InertSustain C8 | First choice with ultra high inertness and high durability | L7 | 2, 3, 5 | 10 | 350 |
| InertSustainSwift C8 | High inertness and high durability C8 column | L7 | 1.9, 3, 5 | 20 | 200 |
| Inertsil C8-4 | Ultra high inertness, High plate count, Low retentivity | L7 | 2, 3, 5 | 10 | 450 |
| Inertsil C8-3 | Strong retentivity, Lower column backpressure, Very inert | L7 | 2, 3, 5, 10 | 10 | 450 |
| Inertsil C8 | Ultra pure silica gel is used | L7 | 5 | 15 | 320 |
| Inertsil C4 | Low retentivity | L26 | 5 | 15 | 320 |
| Inertsil WP300 C8 | Suitable for high molecules | L7 | 5 | 30 | 150 |
| Inertsil WP300 C4 | Suitable for high molecules | L26 | 5 | 30 | 150 |
| InertSustain PFP | Extremely Strong retention of highly polar basic compounds | L43 | 3, 5 | 10 | 350 |
| InertSustain Phenylhexyl | Strong π-π interactions and hydrophobic interactions | L11 | 3, 5 | 10 | 350 |
| InertSustain Phenyl | Extremely strong π-π interactions | L11 | 2, 3, 5 | 10 | 350 |
| Inertsil Ph-3 | Strong π-π interactions | L11 | 2, 3, 5 | 10 | 450 |
| Inertsil Ph | High inertness, Weak π-π interactions | L11 | 5 | 15 | 320 |
| InertSustain Cyano | Ultra inertness and can be used in reversed phase mode | L10 | 3, 5 | 10 | 350 |
Phases providing high retentivity of polar compounds in immobilized water layers on the stationary phase surface in hydrophilic interaction liquid chromatography (HILIC) mode. Our choice of funtionalities allow for an excellent spread of different separation patterns for the analysis of compounds such as sugars.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| InertSustain Amide | First choice for HILIC mode | L68 | 3, 5 | 10 | 350 |
| Inertsil HILIC | Separation of highly polar basic compounds | L20 | 3, 5 | 10 | 450 |
| InertSustain NH2 | First choice for sugar analysis | L8 | 3, 5 | 10 | 350 |
| Inertsil NH2 | Sugar analysis, High retentive in normal phase mode | L8 | 3, 5 | 10 | 450 |
| Inertsil Amide | Increasing retentivity of high polar compounds | L68 | 3, 5 | 10 | 450 |
Phases with ionizable functional groups having high ion exchange capacities are available for both anion and cation exchange chromatography. We feature popular groups for wide method compatibility. In addition, mix-mode columns featuring alkyl groups are available for alternate selectivity.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| Inertsil AX | Anion-exchange column | ― | 5 | 10 | 450 |
| Inertsil CX | Cation-exchange column | L9 | 5 | 10 | 450 |
| InertSustain AX-C18 | Analysis of anionic highly polar compounds | L1, L78 | 3, 5 | 20 | 200 |
High performance phases for retentivity of polar compounds. Our normal phases are available a wide range of pore sizes (10-30 nm) for excellent choice in retentivity and selectivity in analysis of up to large molecules.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| Inertsil Diol | First choice for normal phase mode, For SEC | L20 | 3, 5 | 10 | 450 |
| Inertsil SIL-100A | Ultra pure silica gel with 100Å pore size | L3 | 3, 5 | 10 | 450 |
| Inertsil SIL-150A | Ultra pure silica gel with 150Å pore size | L3 | 5 | 15 | 320 |
| Inertsil WP300 SIL | Ultra pure silica gel with 300Å pore size | L3 | 5 | 30 | 150 |
| Inertsil CN-3 | Can be also used in reversed phase mode | L10 | 3, 5 | 10 | 450 |
| InertSustain Cyano | Ultra inertness and can be used in reversed phase mode | L10 | 3, 5 | 10 | 350 |
Columns featuring diol phases in convenient pore sizes that are compatible with aqueous (GFC) or organic (GPC) size-exclusion chromatography. Our columns are available in smaller inner diameters for solvent saving, low-cost analysis. Serial coupling using different GL Sciences SEC columns allows for analysis of a broad range of molecular weights.
| Column | Features | USP Code | Micron (µm) | Pore Size (nm) | Surface Area (m2/g) |
| Inertsil WP300 Diol | High molecule SEC, Can be also used in normal phase mode | L20, L33 | 5 | 30 | 150 |
| Inertsil Diol | First choice for normal phase mode, For SEC | L20 | 3, 5 | 10 | 450 |
HPLC Column Selection Guide
Separation Mode Selection for Analyte Polarity
Column Selection for Analyte Properties
Column Selection for Vitamins
Column Selection for Sugars and Nucleic acids
Column Selection for Medicines
Column Selection for Organic acids
Column Selection for Amino acids
Column Selection for Proteins and Peptides
Column Selection for Pesticides, Veterinary medicines, Aldehydes and Polymers
HPLC Columns Resources:
- Download GL Sciences 2022 HPLC Columns Catalog — Download
- Download GL Sciences 2022 InertSustain Catalog— Latest HPLC Column Technology Download
- Search Application Notes For HPLC Columns — Search Applications Database
- HPLC Columns’ Specification — Summary of GL Sciences HPLC Column Specifications. Learn More
- HPLC Column Selection Overview — Separation mode selection for analyte polarity & HPLC column selection for analyte properties. Learn More
- HPLC Column Selection By USP — Recommended GL Sciences HPLC Columns based on USP Code. Learn More
- HPLC Column End Fittings — Summary of GL Sciences' end-fitting style & recommended operating pressure. Learn More
- Reversed Phase HPLC Column Selection Guide— Download Reverse Phase HPLC Column Selection Guide. Learn More
- Normal Phase Selection Guide — Download Normal Phase HPLC Column Selection Guide. Learn More
- How to Select a Guard Column — Download guide to select the right guard column. Learn More
- Choosing a Preparative Column — Download Guide to select the right preparative column. Learn More