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HILIC Columns

GL Sciences provides amide, amine and diol phases column phases for separation by HPLC using HILIC (Hydrophilic Interaction Chromatography) mode. GL Sciences HILIC phases feature several generations of development of evolved silica for the analysis of neutral and basic compounds with excellent peak shape. Specific applications of GL Sciences HILIC phases are analysis of sugars and vitamins. Read more

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Picture of INERTSIL NH2 ANALYTICAL COLUMN 7UM 125MM X 4.6MM
INERTSIL NH2 ANALYTICAL COLUMN 7UM 125MM X 4.6MM
SKU# 5020-87044
$640.00
Picture of InertSustain Amide UHPLC PEEK Column, 3 µm, 50 x 4.6 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 3 µm, 50 x 4.6 mm (analytical)
SKU# 5020-87424
$1,210.00
Picture of INERTSIL NH2 ANALYTICAL COLUMN 10UM 250MM X 4.6MM
INERTSIL NH2 ANALYTICAL COLUMN 10UM 250MM X 4.6MM
SKU# 5020-90528
$680.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 150 x 2.1 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 150 x 2.1 mm (analytical)
SKU# 5020-87478
$1,120.00
Picture of INERTSIL NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 150MM X 4.6MM
INERTSIL NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 150MM X 4.6MM
SKU# 5020-89958
$730.00
Picture of Inertsil HILIC Prep Column, 5 µm, 150 x 10 mm
Inertsil HILIC Prep Column, 5 µm, 150 x 10 mm
SKU# 5020-86755
$1,680.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 50 x 4.6 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 50 x 4.6 mm (analytical)
SKU# 5020-87480
$980.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 75 x 2.1 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 75 x 2.1 mm (analytical)
SKU# 5020-88817
$830.00
Picture of INERTSUSTAIN NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 250MM X 4.6MM
INERTSUSTAIN NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 250MM X 4.6MM
SKU# 5020-89957
$870.00
Picture of Inertsil HILIC Prep Column, 5 µm, 150 x 7.6 mm
Inertsil HILIC Prep Column, 5 µm, 150 x 7.6 mm
SKU# 5020-07755
$940.00
Picture of InertSustain Amide UHPLC PEEK Column, 3 µm, 100 x 4.6 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 3 µm, 100 x 4.6 mm (analytical)
SKU# 5020-87425
$1,150.00
Picture of InertSustain Amide UHPLC PEEK Column, 1.9 µm, 100 x 2.1 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 1.9 µm, 100 x 2.1 mm (analytical)
SKU# 5020-88832
$1,260.00
Picture of Inertsil HILIC Prep Column, 5 µm, 100 x 10 mm
Inertsil HILIC Prep Column, 5 µm, 100 x 10 mm
SKU# 5020-86754
$1,330.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 100 x 2.1 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 100 x 2.1 mm (analytical)
SKU# 5020-88818
$830.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 250 x 2.1 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 250 x 2.1 mm (analytical)
SKU# 5020-87479
$1,150.00
Picture of Inertsil HILIC Prep Column, 5 µm, 150 x 14 mm
Inertsil HILIC Prep Column, 5 µm, 150 x 14 mm
SKU# 5020-79063
$2,500.00
Picture of INERTSUSTAIN NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 150MM X 4.6MM
INERTSUSTAIN NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 150MM X 4.6MM
SKU# 5020-89956
$730.00
Picture of Inertsil HILIC Prep Column, 5 µm, 100 x 7.6 mm
Inertsil HILIC Prep Column, 5 µm, 100 x 7.6 mm
SKU# 5020-07754
$890.00
Picture of Inertsil NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
Inertsil NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
SKU# 5020-89953
$730.00
Picture of InertSustain Amide UHPLC PEEK Column, 3 µm, 100 x 2.1 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 3 µm, 100 x 2.1 mm (analytical)
SKU# 5020-87421
$1,150.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 100 x 3.0 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 100 x 3.0 mm (analytical)
SKU# 5020-88823
$830.00
Picture of InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
SKU# 5020-89951
$730.00
Picture of InertSustain Amide UHPLC PEEK Column, 3 µm, 250 x 2.1 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 3 µm, 250 x 2.1 mm (analytical)
SKU# 5020-87423
$1,320.00
Picture of INERTSIL NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
INERTSIL NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
SKU# 5020-90579
$730.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 250 x 4.6 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 250 x 4.6 mm (analytical)
SKU# 5020-87483
$1,150.00
Picture of INERTSUSTAIN NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
INERTSUSTAIN NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
SKU# 5020-90612
$730.00
Picture of INERTSIL AMIDE ANALYTICAL COLUMN 1.9UM 75MM X 3MM
INERTSIL AMIDE ANALYTICAL COLUMN 1.9UM 75MM X 3MM
SKU# 5020-88822
$830.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 150 x 4.6 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 150 x 4.6 mm (analytical)
SKU# 5020-87482
$1,120.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 100 x 4.6 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 100 x 4.6 mm (analytical)
SKU# 5020-87481
$1,120.00
Picture of InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.0 mm (analytical)
InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.0 mm (analytical)
SKU# 5020-89955
$730.00
Picture of Inertsil HILIC Prep Column, 5 µm, 100 x 20 mm
Inertsil HILIC Prep Column, 5 µm, 100 x 20 mm
SKU# 5020-86764
$2,980.00
Picture of INERTSIL NH2 ANALYTICAL COLUMN 7UM 300MM X 3.9MM
INERTSIL NH2 ANALYTICAL COLUMN 7UM 300MM X 3.9MM
SKU# 5020-90529
$730.00
Picture of Inertsil HILIC Prep Column, 5 µm, 50 x 14 mm
Inertsil HILIC Prep Column, 5 µm, 50 x 14 mm
SKU# 5020-79061
$1,450.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 150 x 3.0 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 150 x 3.0 mm (analytical)
SKU# 5020-88824
$880.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 30 x 3.0 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 30 x 3.0 mm (analytical)
SKU# 5020-88820
$760.00
Picture of INERTSIL NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 250MM X 4.6MM
INERTSIL NH2 ANALYTICAL COLUMN WITH 100% CH3CN 3UM 250MM X 4.6MM
SKU# 5020-89959
$870.00
Picture of InertSustain Amide UHPLC PEEK Column, 1.9 µm, 150 x 2.1 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 1.9 µm, 150 x 2.1 mm (analytical)
SKU# 5020-88833
$1,300.00
Picture of Inertsil NH2 HPLC Column, 5 µm, 125 x 4.6 mm (analytical)
Inertsil NH2 HPLC Column, 5 µm, 125 x 4.6 mm (analytical)
SKU# 5020-89602
$640.00
Picture of InertSustain Amide UHPLC PEEK Column, 3 µm, 250 x 4.6 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 3 µm, 250 x 4.6 mm (analytical)
SKU# 5020-87427
$1,320.00
Picture of InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 150 x 4.6 mm (analytical)
InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 150 x 4.6 mm (analytical)
SKU# 5020-89950
$720.00
Picture of Inertsil NH2 Prep Column, 5 µm, 50 x 6.0 mm
Inertsil NH2 Prep Column, 5 µm, 50 x 6.0 mm
SKU# 5020-05552
$630.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 30 x 2.1 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 30 x 2.1 mm (analytical)
SKU# 5020-88815
$760.00
Picture of Inertsil NH2 Prep Column, 5 µm, 100 x 6.0 mm
Inertsil NH2 Prep Column, 5 µm, 100 x 6.0 mm
SKU# 5020-05554
$760.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 50 x 3.0 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 50 x 3.0 mm (analytical)
SKU# 5020-88821
$760.00
Picture of ProteoSil HILIC PEEK Analytical Column 5um, 2.1x250mm
ProteoSil HILIC PEEK Analytical Column 5um, 2.1x250mm
SKU# 5020-42173
$1,277.00
Picture of ProteoSil HILIC Analytical Column 5um, 2.1x75mm
ProteoSil HILIC Analytical Column 5um, 2.1x75mm
SKU# 5020-42221
$580.00
Picture of ProteoSil HILIC Analytical Column 5um, 4.6x150mm
ProteoSil HILIC Analytical Column 5um, 4.6x150mm
SKU# 5020-42228
$638.00
Picture of ProteoSil HILIC Analytical Column 3um, 2.1x75mm
ProteoSil HILIC Analytical Column 3um, 2.1x75mm
SKU# 5020-42234
$630.00
Picture of ProteoSil HILIC PEEK Analytical Column 5um, 2.1x50mm
ProteoSil HILIC PEEK Analytical Column 5um, 2.1x50mm
SKU# 5020-42170
$904.00
Picture of ProteoSil HILIC PEEK Analytical Column 5um, 4.6x150mm
ProteoSil HILIC PEEK Analytical Column 5um, 4.6x150mm
SKU# 5020-42176
$1,142.00

HILIC Column Specification​


Columns

Features

USD Code

Particle Size (µm)

Pore Size (nm)

Surface Area (m2/g)

Carbon Loading (%)

Recommended pH range

InertSustain Amide

First choice column for HILIC mode.

L68

3, 5

10

350

15

2 - 8.5

Inertsil Amide

Effective when the retention of high polar compounds is further strengthened.

L68

3, 5

10

450

18

2 - 7.5

Inertsil HILIC

Effective when the overall retention is to be reduced or when the separation pattern is to be changed.

L20

3, 5

10

450

20

2 - 7.5

InertSustain NH2

First choice column for sugar analysis.

L8

3, 5

10

350

7

2 - 7.5

Inertsil NH2

Effective for intensifying retention in sugar analysis

L8

3, 5

10

450

8

2 - 7.5

 

HILIC Mode Chromatography

As an alternative to reversed-phase chromatography, Hydrophilic Interaction Chromatography (HILIC) was developed for analyses of highly polar compounds. The retention in HILIC mode usually increases with increasing concentration of an organic compound. Moreover, the stability is improved by adding a basic solvent (such as ammonium acetate) to the eluent.

 

Comparison of Separation Mode between Reversed Phase and HILIC Columns

Comparison of Separation Mode between Reversed Phase and HILIC Columns
 

 Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase

Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase
 

HILIC Column Selection

HILIC Column Selection flowchart
 

HILIC Columns Resources:​

  • Download GL Sciences 2022 HILIC Catalog —  Download & Discover GL Sciences' HILIC Columns
  • Search Application Notes For HILIC Columns — Search  Applications Database
  • HPLC HILIC Columns’ Specifications and Selection Guide — Summary of GL Sciences HILIC Columns Specifications and guide to select. Learn More
  • HPLC Column Selection By USP  — Recommended GL Sciences HPLC Columns based on USP Code. Learn More
  • HPLC Column End-fittings  — Summary of GL Sciences' end-fitting style & recommended operating pressure. Learn More
  • How to Select a Guard Column  — Download guide to select the right guard column. Learn More
  • Choosing Preparative Column  — Download Guide to select the right preparative column. Learn More
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