GL Sciences HPLC Column Catalog

Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C18 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise con...

InertSustain AQ-C18 columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goals in developing reversed phase methods. The optimization of bonding of the C18 groups at equal distance to the silica gel enable InertSustain AQ-C18 columns to offer significant retention for highly polar comp...

Increasing Efficiency, Throughput and Productivity InertSustainSwift C18 columns are designed to deliver rapid separations with symmetric peaks to minimize analysis time as much as possible. InertSustainSwift C18 is also ideal for LC-MS and LC-MS/MS methods which offer highly sensitive results and enables MS compatible buffers to be u...

Inertsil ODS-3V offers all of the outstanding chromatographic benefits of Inertsil ODS-3 with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance. Each Inertsil ODS-3V is delivered with a Manufacturer‘s Validation Certificate showing the detailed results of every QA ...

Inertsil ODS-3 is still GL Sciences’ most popular phase and continues to be used widely and reliably for long-established methods in pharmaceutical, and contract research labs. Inertsil ODS-3 columns have a relatively strong retentivity compared to commercially available ODS columns. In addition, the introduction of higher surface area si...

Ultra High Retentivity Ideal for Separation of Basic Molecules & its Related Substances, Process Impurities Inertsil ODS-HL employs a highly inert packing material which provides pure hydrophobic interaction between analytes without generating any secondary interaction delivering sharp peaks. High-Density bonding of C18 phase delivers ...

Inertsil ODS-4 delivers the same extreme inertness to any type of compounds just like InertSustain C18 along with unprecedented stability under 100 % aqueous mobile phases for qualitative and quantitative analysis. However, as the base silica gel and carbon loading are different on Inertsil ODS-4, differences in selectivity can be observe...

Inertsil ODS-4 columns have proven superior worldwide for analysis of strong pharmaceutical bases, acids, chelating compounds, and zwitterions. The long-awaited validated Inertsil ODS-4V has now been added to our product lineup. Each Inertsil ODS-4V is delivered with a Manufacturer’s Validation Certificate showing the detailed results ...

Inertsil ODS-SP is super base deactivated and optimally bonded to retain hydrophilic compounds without excessive retention of hydrophobic compounds achieving better separations faster than before. As the carbon load of Inertsil ODS-SP is relatively low, it is compatible with 100 % aqueous eluents and offer faster equilibration of column f...

Inertsil ODS columns are general purpose, reversed phase C18 columns available in 5 μm and 10 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 1986. We recommend InertSustain C18 or Inertsil ODS-4 columns for all new method development. Specifications • Silica : Spherical Silica Gel • Parti...

Inertsil ODS-2 columns have a pore size of 150 Å offering symmetric peaks for bases, acids with low pressure. When Inertsil ODS-2 was introduced in 1987s, this HPLC transformed the entire industry. Inertsil ODS-2 was the first HPLC phase created using ultra high purity silica, which produced superior base deactivation. Inertsil ODS-2 is o...

The same base silica gel and bonding technology that is used for Inertsil ODS-3 is also employed for Inertsil C8-3. The difference between the two phases is just the length of the hydrocarbon ligands. 10 μm particle size columns are also available to meet the requirement of various pharmacopeia methods. We recommend InertSustain C8 column...

Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C18 group. The embedded polar group makes the C18 phase stable in 100 % aqueous eluents without "phase collapse." This phase is also extremely "base deactivated" and provides superior peak shape for acids and bases in organic eluents as well a...

GL Sciences offers a polymerically bonded ODS-P phase which provide high steric selectivity for separation of planar and non-planar compounds. nertsil ODS-P columns are also ideal for the HPLC analysis of 16 PAH compounds, listed as target pollutants by the US EPA. Specifications • Silica : 3 Series High Purity Silica Gel • Particle ...

Inertsil WP300 C18 (wide pore size of 300 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible separations of proteins and peptides. The results of GL Sciences’ original end-capping technique provide high efficiency and good peak shape for proteins an...

Inertsil C4 columns have a pore size of 150 Å and is recommended for rapid analysis of highly hydrophobic compounds such as fat-soluble vitamins. Specifications • Silica : 2 Series High Purity Silica Gel • Particle Size : 5 μm • Surface Area : 320 m2/g • Pore Size : 150 Å (15 nm) • Pore Volume : 1.20 mL/g • Functional Group : But...

Inertsil C8 columns have a pore size of 150 Å and it is recommended for rapid analysis of highly hydrophobic compounds. We recommend InertSustain C8 or Inertsil C8-4 columns for all new method development. Specifications • Silica : 2 Series High Purity Silica Gel • Particle Size : 5 μm • Surface Area : 320 m2/g • Pore Size : 150 ...

Many chromatographers prefer a C8 column when an ODS phase shows excessive retention values. Inertsil C8-4 provides the same separation pattern (selectivity) and extreme inertness to any type of compounds just like Inertsil ODS-4, which enables easy method transfer from ODS-4 to C8-4 while other commercially available ODS and C8 columns c...

Inertsil WP300 C4 is a butyl group bonded phase utilizing silica gel with wide pores (300 Å). Also, an optimal polar group is embedded between silica surface and butyl group, which reduces adsorption of basic compounds and fat-soluble proteins. Inertsil WP300 C4 is recommended for the analysis of large, highly fat-soluble proteins and pep...

Inertsil WP300 C8 (wide pore size of 300 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible rapid separations of proteins and peptides. Inertsil WP300 C8 delivers rapid analysis with sharper peaks compared to Inertsil WP300 C18. Specifications •...

Inertsil Ph has phenethyl groups bonded to silica gel which offers weak pi-pi interactions. As it is modified with phenethyl groups, hydrophobic interactions between alkyl chain and analytes play an important role in separation as well as π-π interactions. To change the selectivity or elution pattern drastically, InertSustain Phenyl is re...

Just like InertSustain Phenyl, Inertsil Ph-3 has phenyl groups directly bonded to the silica gel which provides pure reverse phase characteristics that are critical to resolving highly polar compounds like acidic and basic pharmaceuticals. The near perfect phenyl phase coverage on this material results in symmetric, narrow peaks for even ...

Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime. ...

HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when...

Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous ana...

Inertsil CN-3, Cyanopropryl groups bonded to Silica gel with high density. And it is not only increase difference recognition of hydrophilicity, but also increase the durability. It is difficult to increase the both performance with other previous cyanopropryl columns. Inertsil CN-3 can be cleaned with 100 % aqueous solvent because non-sp...

Inertsil AX has diethylamino groups bonded to silica gel by an alkyl chain. The diethylamino groups offer anionic functions required for anion exchange chromatography. It is mainly used for analyses of acidic compounds. Conventional ion-exchange columns used to show inconsistent results from lot to lot. However, Inertsil AX is manufacture...

The Inertsil CX Cation Exchange HPLC Column has benzenesulfonyl groups bonded to silica gel by an alkyl chain. The sulfonyl groups at the end of the structure offer cationic functions required for the cation exchange chromatography. It is mainly used for analyses of basic compounds. Inertsil CX is manufactured under strict quality control...

Inertsil Diol column has characteristics of dihydroxypropyl group bonded phase. It shows unique selectivity normal phase mode. The separation mechanism of diol column is featured by hydrogen bonding interactions between diol groups and polar compounds. Diol columns provide an alternative selectivity to silica columns often with increased ...

Inertsil SIL-100A is a pure silica gel column available in normal phase mode. Because of the high quality of its silica gel, Inertsil SIL-100A achievs separation with sharp peaks and provides high column-to-column reproducibility. This excellent silica gel ideally designed for HPLC is the basis for “Inertsil 3-series” of GL Scineces. GL S...

Inertsil SIL-150A is ultra pure silica gel column, and this ultra pure silica gel contains very low level of metal impurities and is durable and free from dents and cracks which can cause premature column failure. Compared to Inertsil SIL-100A, the silica’s surface area is smaller (320 m²/ g). Retentivity of Inertsil SIL-150A is weaker th...

Specifications • Base Material : 3 Series High Purity Silica Gel • Particle Size : 3 μm, 5 μm • Surface Area : 450 m²/g • Pore Size : 100 Å (10 nm) • Pore Volume : 1.05 mL/g • Functional Group : Octadecyl • End-capping : Yes • Carbon Loading : 15 % • USP Code : L1

Inertsil WP300 Diol has dihydroxypropyl group bonded to silica gel with pore size 300 Å and is capable of analyzing large molecules. Like Inertsil Diol, Inertsil WP300 Diol can be used for both aqueous SEC (Size Exclusion Chromatography) and organic SEC. Also, as a diol column, Inertsil WP300 Diol can be used in both normal phase and reve...

Inertsil WP300 SIL is pure silica gel phase with wide pores(300 Å). It is available for analysing compounds including large molecules. As the pore becomes wider, the surface area of silica gel is smaller. Since the interactions between the analyte and silica gel occur on the sIlica surface, smaller surface area means less interactions and...

InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phase...

InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by Anion Exchange group works. By controlling the amount of Anion Exchange group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS. Specificat...

InertSustain C8 is an octyl group (C8) bonded column delivering the same extreme inertness to any type of compounds just like InertSustain C18, which enables rapid analysis of highly hydrophobic compounds delivering symmetric peaks at a wide range of pH. Specifications • Silica : High Purity ES Silica Gel • Particle Size : 2 μm, 3 μm...

In general, the stability and reproducibility of the Cyano phase available in the market are poor. Many batch-to-batch or lot-to-lot reproducibility issues are occurring at many laboratories. The InertSustain Cyano columns were developed to resolve these problems and are designed using the most modern LC column technology available provid...

InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of ...

InertSustain PFP columns are bonded with pentafluorophenyl groups to our newly developed ES silica gel, which delivers unique separation patterns with excellent peak shape and sensitivity. The chromatographic difference stems from the fact that the PFP phases provide interactions such as π-π, dipole, hydrogen bonding, and ionic interactio...

InertSustain Phenyl delivers an extremely unique reverse phase characteristics that are critical to resolving compounds that could not be separated on a C18 or C8 phase. InertSustain Phenyl provides not only pi-pi interactions, but also hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. A...

InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6- carbon) linker and is densely bonded to our newly developed ES silica gel delivering complementary selectivity to straight alkyl-chain columns, but with industry leading inertness, lot-to-lot reproducibility and low back press...

The 200 Å pore size silica creates the opportunity to separate compounds having a molecular weight from small to large molecules. In addition, the usage of highly inert packing material packed into a new Steel-Coated-PEEK hardware deliver excellent peak shapes for various analytes without any adsorption. Specifications • Base Material...

InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C18, which is ideal for analyzing low polarity analytes. In addition, the optimized 200Å pore size silica enables to analyze and retain peptides and oligonucleotides which have a molecular weig...

Maximizing all the benefits and advantages of monolithic technology, MonoCap High Resolution and High Resolution Ultra are appropriate for the efficient separation of peptides and protein digests. MonoCap High Resolution provide extremely high efficiency, delivering over 200,000 plates for a 2,000 mm length column. The High Resolution...

MonoCap C18 Nano-flow produces higher number of theoretical plates compared to a totally porous particle type capillary column packed with a 3µm packing material. It can be operated at a wide range of flow rate with low back pressure and achieve very high sensitive results in Nano-LC-ESI/MS applications. Columns are protected by either me...

MonoCap Trap columns have a relatively big throughpore and workable at a high flow rate such as 10 μL/min. This benefit makes MonoCap Trap columns to be appropriate for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection. End-fittings are 1/16“ (10-32 UNF). 1/32“...

MonoSelect C18 for HTS is based on monolithic silica technology. The very low flow resistance and the excellent separation performances make it suitable for High-Throughput Screenings (HTS). Due to its metal-free hardware, sharp peaks can be obtained also when analyzing metal chelators. The particular structure of MonoSelect C18 for HTS p...

MonoSelect nPEC uses nanoparticle exclusion chromatography (nPEC). It can rapidly separate nano-particles and free-low molecule compounds. Specifications • Silica:High Purity Monolithic Silica Gel • Through Pore Size: 1 µm • Surface Area: 340 m2／g • Pore Size (Moso-pore Size): 110 Å (11 nm) • Functional Group: Hydrophilic Pol...

MonoSelect RP-mAb is an HPLC column specialized for monoclonal antibody analysis. Monolithic silica delivers “Strong Retentivity and Low Adsorption”. Sharp peaks can perform even in large molecular protein analysis such like antibodies. Specifications • Base Material: Highly Pure Monolithic Silica • Through Pore Size: 1 µm • Mos...