Application Notes

When the pH of mobile phase is changed in the analysis of reverse phase mode on HPLC, the dissociation / non-dissociation state changes depending on the compound, so the retention time changes. Normally, the retention time is longer in non-dissociated state and shorter in dissociated state. This time, using Inertsil WP300 C18, we analyzed the basic compound saccharin and the acidic compound phthalic anhydride standard, and found that the elution order changed under acidic and neutral conditions. We also report that it was estimated that phthalic anhydride in the sample was partially changed to phthalic acid.

Glucosamine is an amino sugar in which one of the hydroxy groups of glucose is replaced with an amino group, and is often used in health foods. At the 10th Edition Food Additive Official Formulation Study Group (3rd), a draft ingredient standard using liquid chromatography as a quantification method for glucosamine was decided. This time, we would like to introduce the analysis of glucosamine that complies with the draft component standard using the Hitachi HPLC system Chromaster.

Malic acid is one of the organic acids and is widely present in foods and living organisms. Analysis of the malic acid standard reagent confirmed the phenomenon that two peaks appeared depending on the detection method. This time, we analyzed malic acid using a diode array detector, and it was estimated that the impurity contained in malic acid was fumaric acid.

ß-Cryptoxanthin is a carotenoid pigment found in the edible part of the fruit Citrus unshiu. In addition to its function as provitamin A, which is mainly metabolized to the essential nutrient vitamin A, it is also known to have a excellent antioxidant properties derived from the highly conjugated double bonds in the molecular framework. ß-Cryptoxanthin is considered to be effective in preventing lifestyle-related diseases and in reducing the incidence of osteoporosis. Many foods, supplements, soft drinks, etc. are commercially available that take advantage of these beneficial effects. In this application note, separation and analysis was made with reference to the food sanitation inspection guidelines, and the JAS amendment.

Assuming a fire site where fuels such as gasoline, kerosene, and light oil were used, we used HandyTD TD265 to perform a concentration analysis of the components that volatilize from the recovered material after combustion. In the recovered material, components derived from the fuel used for combustion were detected.

InertCap ProGuard is a capillary column with an integrated guard column, which protects the column from impurities that may be present in the sample.? With InertCap ProGuard, the life of the capillary column can be extended, even when analyzing samples containing large quantities of contamination.

The 17th revision of Japanese Pharmacopoeia stipulates a method using gas chromatography as a method for measuring the alcohol content in products. This time, we will report the results of pretreatment and analysis with reference to "Alcohol number measurement method" test for Pharmacopoeia.