Cyromazine is triazine based molecule that is used as an insect growth inhibitor for the protection of livestock and poultry houses. Standard values have been set for its use in veterinary drugs, and a notification method using HPLC has been issued. In this study, extraction and purification were performed with a mixed-mode polymer SPE (InertSep MCX FF) using a method based on the notification test method from the Ministry of Health, Labor and Welfare and literature examples*, and analysis was performed using HPLC (detection at UV 240nm) and LC-MS/MS.