Need help? 1-310-984-1946 or Live Chat
English
English
Contact Us
Catalogs
Quick Order
Contact Us
Catalogs
Quick Order
English
English
Search
You have no items in your shopping cart.

HILIC Columns

GL Sciences provides amide, amine and diol phases column phases for separation by HPLC using HILIC (Hydrophilic Interaction Chromatography) mode. GL Sciences HILIC phases feature several generations of development of evolved silica for the analysis of neutral and basic compounds with excellent peak shape. Specific applications of GL Sciences HILIC phases are analysis of sugars and vitamins. Read more

Grid List
Filters
per page
InertSustain Amide HILIC HPLC Columns
InertSustain Amide
InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phase...
From $280.00
Inertsil Amide HILIC HPLC Columns
Inertsil Amide
Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime.
From $280.00
Inertsil HILIC
Inertsil HILIC
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when...
From $280.00
InertSustain NH2
InertSustain NH2
InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of ...
From $280.00
Inertsil NH2 HILIC Amino HPLC Columns
Inertsil NH2
Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous ana...
From $280.00
ProteoSil HILIC
ProteoSil HILIC
Modified with amide groups, the ProteoSil HILIC column excels in the separation of highly hydrophilic compounds, peptides, glycans, and oligonucleotides.
From $192.00
Picture of Inertsil Amide HPLC Column, 5 µm, 150 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 150 x 2.1 mm (analytical)
SKU# 5020-07805
$600.00
Picture of Inertsil Amide HPLC Column, 5 µm, 250 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 250 x 2.1 mm (analytical)
SKU# 5020-07806
$640.00
Picture of Inertsil Amide HPLC Column, 5 µm, 150 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 150 x 3.0 mm (analytical)
SKU# 5020-07815
$600.00
Picture of Inertsil Amide HPLC Column, 5 µm, 250 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 250 x 3.0 mm (analytical)
SKU# 5020-07816
$640.00
Picture of Inertsil Amide HPLC Column, 5 µm, 150 x 4.6 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 150 x 4.6 mm (analytical)
SKU# 5020-07835
$600.00
Picture of Inertsil Amide HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
SKU# 5020-07836
$640.00
Picture of InertSustain NH2 HPLC Column, 5 µm, 300 x 3.9 mm (analytical)
InertSustain NH2 HPLC Column, 5 µm, 300 x 3.9 mm (analytical)
SKU# 5020-89979
$790.00
Picture of InertSustain Amide UHPLC PEEK Column, 3 µm, 150 x 2.1 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 3 µm, 150 x 2.1 mm (analytical)
SKU# 5020-87422
$1,350.00
Picture of InertSustain Amide UHPLC PEEK Column, 1.9 µm, 50 x 2.1 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 1.9 µm, 50 x 2.1 mm (analytical)
SKU# 5020-88831
$1,150.00
Picture of INERTSUSTAIN NH2 PREPARATIVE COLUMN 5UM 250MM X 30MM
INERTSUSTAIN NH2 PREPARATIVE COLUMN 5UM 250MM X 30MM
SKU# 5020-89998
$9,010.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 100 x 2.1 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 100 x 2.1 mm (analytical)
SKU# 5020-87477
$1,250.00
Picture of InertSustain Amide HPLC Column, 1.9 µm, 150 x 2.1 mm (analytical)
InertSustain Amide HPLC Column, 1.9 µm, 150 x 2.1 mm (analytical)
SKU# 5020-88819
$880.00
Picture of InertSustain Amide UHPLC PEEK Column, 3 µm, 50 x 2.1 mm (analytical)
InertSustain Amide UHPLC PEEK Column, 3 µm, 50 x 2.1 mm (analytical)
SKU# 5020-87420
$1,050.00
Picture of INERTSIL NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
INERTSIL NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
SKU# 5020-90579
$750.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 250 x 4.6 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 250 x 4.6 mm (analytical)
SKU# 5020-87483
$1,550.00
Picture of INERTSUSTAIN NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
INERTSUSTAIN NH2 ANALYTICAL COLUMN 10UM 300MM X 3.9MM
SKU# 5020-90612
$790.00
Picture of INERTSIL AMIDE ANALYTICAL COLUMN 1.9UM 75MM X 3MM
INERTSIL AMIDE ANALYTICAL COLUMN 1.9UM 75MM X 3MM
SKU# 5020-88822
$800.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 150 x 4.6 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 150 x 4.6 mm (analytical)
SKU# 5020-87482
$1,350.00
Picture of InertSustain Amide HPLC PEEK Column, 5 µm, 100 x 4.6 mm (analytical)
InertSustain Amide HPLC PEEK Column, 5 µm, 100 x 4.6 mm (analytical)
SKU# 5020-87481
$1,250.00
Picture of InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.0 mm (analytical)
InertSustain NH2 with 100% CH3CN HPLC Column, 5 µm, 250 x 4.0 mm (analytical)
SKU# 5020-89955
$740.00
Picture of Inertsil HILIC Prep Column, 5 µm, 100 x 20 mm
Inertsil HILIC Prep Column, 5 µm, 100 x 20 mm
SKU# 5020-86764
$3,330.00
Picture of INERTSIL NH2 ANALYTICAL COLUMN 7UM 300MM X 3.9MM
INERTSIL NH2 ANALYTICAL COLUMN 7UM 300MM X 3.9MM
SKU# 5020-90529
$750.00
Picture of Inertsil HILIC Prep Column, 5 µm, 50 x 14 mm
Inertsil HILIC Prep Column, 5 µm, 50 x 14 mm
SKU# 5020-79061
$1,450.00
Picture of InertSustain Amide HPLC Column, 3 µm, 250 x 4.0 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 250 x 4.0 mm (analytical)
SKU# 5020-88748
$760.00
Picture of InertSustain Amide HPLC Column, 3 µm, 30 x 4.6 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 30 x 4.6 mm (analytical)
SKU# 5020-88750
$580.00
Picture of InertSustain Amide HPLC Column, 3 µm, 50 x 4.6 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 50 x 4.6 mm (analytical)
SKU# 5020-88751
$580.00
Picture of InertSustain Amide HPLC Column, 3 µm, 75 x 4.6 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 75 x 4.6 mm (analytical)
SKU# 5020-88752
$640.00
Picture of InertSustain Amide HPLC Column, 3 µm, 100 x 4.6 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 100 x 4.6 mm (analytical)
SKU# 5020-88753
$640.00
Picture of InertSustain Amide HPLC Column, 3 µm, 125 x 4.6 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 125 x 4.6 mm (analytical)
SKU# 5020-88754
$740.00
Picture of InertSustain Amide HPLC Column, 3 µm, 150 x 4.6 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 150 x 4.6 mm (analytical)
SKU# 5020-88755
$740.00
Picture of InertSustain Amide HPLC Column, 3 µm, 250 x 4.6 mm (analytical)
InertSustain Amide HPLC Column, 3 µm, 250 x 4.6 mm (analytical)
SKU# 5020-88756
$760.00
5020-88693 InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.05 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.05 mm
SKU# 5020-88693
$880.00
5020-88694 InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.05 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.05 mm
SKU# 5020-88694
$970.00
5020-88696 InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.075 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.075 mm
SKU# 5020-88696
$880.00
5020-88697 InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.075 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.075 mm
SKU# 5020-88697
$970.00
5020-88699 InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.1 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.1 mm
SKU# 5020-88699
$880.00
5020-88700 InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.1 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.1 mm
SKU# 5020-88700
$970.00
5020-88702 InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.2 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 50 x 0.2 mm
SKU# 5020-88702
$880.00
5020-88703 InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.2 mm
InertSustain Amide Capillary EX-Nano Column, 5 µm, 150 x 0.2 mm
SKU# 5020-88703
$970.00
5020-88685 InertSustain Amide Capillary EX Column, 5 µm, 50 x 0.3 mm
InertSustain Amide Capillary EX Column, 5 µm, 50 x 0.3 mm
SKU# 5020-88685
$880.00
5020-88686 InertSustain Amide Capillary EX Column, 5 µm, 150 x 0.3 mm
InertSustain Amide Capillary EX Column, 5 µm, 150 x 0.3 mm
SKU# 5020-88686
$930.00
5020-88687 InertSustain Amide Capillary EX Column, 5 µm, 50 x 0.5 mm
InertSustain Amide Capillary EX Column, 5 µm, 50 x 0.5 mm
SKU# 5020-88687
$880.00
5020-88688 InertSustain Amide Capillary EX Column, 5 µm, 150 x 0.5 mm
InertSustain Amide Capillary EX Column, 5 µm, 150 x 0.5 mm
SKU# 5020-88688
$930.00
5020-88689 InertSustain Amide Capillary EX Column, 5 µm, 50 x 0.7 mm
InertSustain Amide Capillary EX Column, 5 µm, 50 x 0.7 mm
SKU# 5020-88689
$880.00

HILIC Column Specification​


Columns

Features

USD Code

Particle Size (µm)

Pore Size (nm)

Surface Area (m2/g)

Carbon Loading (%)

Recommended pH range

InertSustain Amide

First choice column for HILIC mode.

L68

3, 5

10

350

15

2 - 8.5

Inertsil Amide

Effective when the retention of high polar compounds is further strengthened.

L68

3, 5

10

450

18

2 - 7.5

Inertsil HILIC

Effective when the overall retention is to be reduced or when the separation pattern is to be changed.

L20

3, 5

10

450

20

2 - 7.5

InertSustain NH2

First choice column for sugar analysis.

L8

3, 5

10

350

7

2 - 7.5

Inertsil NH2

Effective for intensifying retention in sugar analysis

L8

3, 5

10

450

8

2 - 7.5

 

HILIC Mode Chromatography

As an alternative to reversed-phase chromatography, Hydrophilic Interaction Chromatography (HILIC) was developed for analyses of highly polar compounds. The retention in HILIC mode usually increases with increasing concentration of an organic compound. Moreover, the stability is improved by adding a basic solvent (such as ammonium acetate) to the eluent.

 

Comparison of Separation Mode between Reversed Phase and HILIC Columns

Comparison of Separation Mode between Reversed Phase and HILIC Columns
 

 Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase

Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase
 

HILIC Column Selection

HILIC Column Selection flowchart
 

HILIC Columns Resources:​

  • Download GL Sciences 2022 HILIC Catalog —  Download & Discover GL Sciences' HILIC Columns
  • Search Application Notes For HILIC Columns — Search  Applications Database
  • HPLC HILIC Columns’ Specifications and Selection Guide — Summary of GL Sciences HILIC Columns Specifications and guide to select. Learn More
  • HPLC Column Selection By USP  — Recommended GL Sciences HPLC Columns based on USP Code. Learn More
  • HPLC Column End-fittings  — Summary of GL Sciences' end-fitting style & recommended operating pressure. Learn More
  • How to Select a Guard Column  — Download guide to select the right guard column. Learn More
  • Choosing Preparative Column  — Download Guide to select the right preparative column. Learn More
Filters
Refine
×
Column or Guard
Brand
Column format
Phase
Functional group
Particle size
Length
Inner diameter
Mode
Pore size
End capping
Carbon loading
pH Range
Max Operating Pressure
Target Biomolecule
USP Code
Categories
    Contact Info
    Information
    Customer Service
    Copyright © 2024 GL Sciences Inc.. All rights reserved.
    ;;